General Immunohistochemistry - This forum is open for all to discuss anything related to Immunohistochemistry and Immunofluorescence techniques.
General In Situ Hybridization - This forum is open for all to discuss anything related to In Situ Hybridization (ISH) and Fluorescent In Situ Hybridization (FISH) techniques.
Immunohistochemistry - In Situ Hybridization Topics / Posts:
Adobe Acrobat Reader - To read some of the files hosted on this web site, you may nead Adobe Acrobat Reader.
Adobe Reader 6.0 is the newest version of the familiar Adobe Acrobat Reader software. It also replaces Adobe Acrobat eBook Reader, software for viewing high-fidelity eBooks on your notebook or desktop computer.
Immunohistochemistry vade mecum - An excellent piece of work by Dr. Paul W. Bishop
You can search for tumours to find out their pattern of immunoreactivity or look for individual antibodies to check their specification. There are links to all the references through PubMed. This amazing programme is small, easy to install and runs on any PC.
To download the latest version, go to author's Home Page
Technical Immunohistochemistry - TECHNICAL IMMUNOHISTOCHEMISTRY:
Achieving Reliability and Reproducibility of
Immunostains. By Rodney T. Miller, M.D.
Director of Immunohistochemistry
ProPath Laboratory, Inc.
Immunohistochemistry - In Situ Hybridization Web Links:
35S-labeled probe - In Situ Hybridization using 35S-labeled probe, Detection by emulsion or film autoradiography. Frozen Sections.
A Concise Guide to cDNA Microarray Analysis - Standard protocol including Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. This is a 27-page PDF file.
(The Institute for Genomic Research)
A Novel Quality Control Slide for Quantitative Immunohistochemistry Testing - We introduce a novel quality control technology that may improve intra- and interlaboratory immunohistochemistry (IHC) standardization. The technology involves the creation of standardized antibody targets that are attached to the same slides as the patient sample. After IHC staining, the targets turn the same color as the stained cells or tissue elements. Unlike current clinical practice, our proposed targets are neither cells nor tissue sections.
Abazyme - A variety of antibodies including anti-human cytokine and anti-human CD antigens antibodies
Abcam - Mission:"...to build the largest catalog of the best antibodies in the world"
Abgent - Abgent develops innovative reagents and technologies to profile post-translational modifications of the proteome.
Abkem Iberia - Polyclonal antibodies for food and environmental contaminants, allergens, and growth promoters
Abnova Corporation - Abnova is the world largest monoclonal antibody manufacturer. We have a capacity of generating 500 mouse monoclonal antibodies per month. Rather than the traditional method of monoclonal antibody production, Abnova is taking a genomic/proteomic approach for the antibody development. Our goal is to have at least one antibody to every human expressed gene in human genome. We manufacture all our products, recombinant proteins and antibodies, in house in our stat-of-art facility in Taiwan. In most cases, we have multiple clones for our monoclonal antibodies, and their access is available to our industrial partners and collaborators from the academic institutions.
Academy Bio-Medical Co - Specializing in Human Lipoproteins, Apolipoproteins and Polyclonal Antibodies.
Accurate Chemical & Scientific - Accurate has specialized in products for basic and R&D research as well as routine laboratory work.
Polyclonal and monoclonal antibodies
Acris - Where ever your research interests may be and what ever animal model you work with you will have a good chance to find what you need.
ACTIgen - Develops and markets rProteinL for detection and purification of antibodies
Active Motif - Kits and reagents for monitoring phosphorylation and other events that change the activation state of transcription factors, signalling proteins and receptors.
ADI - Custom peptides and antbodies. They also sell a unique collection of antibodies to receptors, transporters, neurobiologicals, and much more.
Advanced Biotechnologies Inc - Supplier of monoclonal antibodies to the following viruses: HIV-1, SIV, HSV-1, HSV-2, CMV, EBV, VZV, HHV-6, HHV-7, HHV-8 and polyclonal antibodies to HIV-1, HTLV-1, HHV-8 vIL-6, and SIV
Advanced Targeting Systems - An international supplier of neuroendocrine and neuroscience polyclonal and monoclonal antibodies. ATS also provides agents for the targeting and elimination of specific cell types
Affibody - Affibody is a biotech company that uses innovative protein-engineering technologies for the development of products for biotherapeutics and biotechnology applications. The Affibody® molecule, which is a key component of the company’s technology, mimics a monoclonal antibody in many ways, and its properties make it a superior choice for many applications.
Affinity BioReagents - ABR—Affinity BioReagents, a privately held corporation, offers over 2,600 industry leading research reagents and custom antibody production services to the worldwide research community. The ABR product portfolio includes primary and secondary antibodies, proteins, peptides and viral expression kits supporting 34 distinct research areas. Cutting-edge antibody production services allow researchers the flexibility to create a custom antibody to meet their specific research needs.
Agdia - Easy-to-use test kits, reagents and laboratory testing services for agricultural diagnostics
AgriSera - Antibodies to mammalian, insect and bacterial antigens as well as antibodies in the area of photosynthetic research.
Ah Receptor (AhR) - Detection of Ah Receptor (AhR) in Formalin-Fixed, Paraffin-Embedded Rat Tissue
Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates - We report here the superior, largely unparalleled fluorescence emission and photostability of conjugates of a new series of sulfonated compounds named Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes. The number in each of their names refers to the approximate excitation maxima of the dyes, which nearly match the principal wavelength of several commonly used excitation sources. All of the Alexa dyes exhibit pH-insensitivity over a very broad range, and those that absorb at wavelengths greater than 480 nm have the high molar extinction coefficient typical of fluoresceins or rhodamines.
Alexis - Apoptosis, Cell Cycle & DNA Transactions, Combinatorial Chemistry Reagents, Cytoskeletal Research, Growth Factors, Cytokines & Chemokines, Immunology & Cancer Research, Neurochemicals, Nitric Oxide & Oxidative Stress, Signal Transduction.
Alomone Labs - Supplying innovative biochemicals which serve the neuroscience research community.
Alpha Diagnostic International - Alpha Diagnostic Intl., Inc., (ADI) located in San Antonio, Texas, USA, is one of the major providers of custom services such as peptides synthesis, and polyclonal antibodies. ADI maintains an USDA inspected and approved, animal facility. We have NIH/OPRR Assurance on file. Other antibody related services (affinity purification, conjugation to enzymes, dyes, fluorochromes, etc, ELISA, Western blots) are also provided.
ALT Inc - Novel, site specific nucleotide based photoaffinity reagents for labeling of antibodies
American Qualex - Labeled primary and secondary antibodies in addition to antibodies for signal transduction.
Amplification Methods to Increase the Sensitivity of In Situ Hybridization - Here we outline the principles of tyramide signal amplification using the catalyzed reporter deposition (CARD) technique, present practical suggestions to efficiently enhance the sensitivity of ISH with CARD, and discuss some applications and possible future directions of in situ nucleic acid detection using such an amplification strategy.
Amylase - Identification of Amylase (C-20) in Formalin-Fixed, Paraffin-Embedded Rat Tissue
An Optimized Method for ISH with Signal Amplification That Allows the Detection of Rare mRNAs - Using biotinyl–tyramide and streptavidin conjugated to alkaline phosphatase (AP) to introduce an additional round of amplification, we have now achieved a considerable enhancement of signal intensity relative to standard chromogenic ISH detection systems. We term this protocol TSA-AP (tyramide signal amplification using AP). In addition, we have optimized critical parameters to improve hybridization with digoxigenin (DIG)-labeled probes and to reduce nonspecific binding of probes, both strategies being essential for successful detection of rare mRNAs.
AnaSpec - Life science company providing innovative products (peptides, antibodies, fluorescent reagents, assay kits, synthesis resins and reagents) and custom manufacturing. AnaSpec offers a broad product line of these biochemicals and reagents for basic research, high-throughput screening and drug discovery. In conjunction, AnaSpec provides premier custom services including peptide synthesis, antibody production, and assay development.
Anaspec - An international manufacturer of antibodies in the areas of apoptosis, cancer research, and signal transduction; and secondary antibodies.
Anawa - Custom antibody production and a large selection of antibodies
Anderson Lab In Situ Hybridization Protocols - In Situ Hybridization of Frozen Sections, Whole Mount In Situ Hybridization, In Situ Hybridization on Cultured Cells and Additional Techniques
AngioBio - Provider of reagents for angiogenesis and lymphangiogenesis research, including many antibodies.
Anogen - Antibodies to cytokines, viral proteins, tumor markers and growth factors; and custom monoclonal antibody services.
Antibodies - Structure and Sequence - This page attempts to summarise useful information on antibody structure and sequence. It provides a query interface to the Kabat antibody sequence data, general information on antibodies and crystal structures and links to other antibody-related information.
Antibodies Incorporated - Antibodies Incorporated is owned and operated by employees who have many years of experience in animal care, antiserum production, immunochemistry and related services applicable to aiding the academic or industry research scientist to save time and money in pursuit of their scientific goals. Having developed and manufactured our own diagnostic kits for many years has given us experience that is highly regarded in developing new assays and for contract manufacturing.
Antibody Directory - We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive.
Antibody Purification Handbook - The purpose of this handbook is to present the most effective and most frequently used
strategies for sample preparation and purification of the many different forms of antibodies
and antibody fragments used in the laboratory.
Antibody Staining of Drosophila Adult Retina - The following protocol has been adapted from Larry Zipursky's lab. It detects antigen in retinas that have been sectioned in plastic.
AntibodyShop - Large selection of monoclonal antibodies available online. The main antigens are: mannan binding lectin, complement system components, blood coagulation cascade components, immunoglobulins, albumins, cytokines, hormones, enzymes, haptens, human plasma proteins, and bacterial antigens.
Antigen Retrieval in Prion Protein Immunohistochemistry - Many different protocols for the detection of prions in brain tissue have been used. Thus far, picric and/or formic acid, steam autoclaving at 121C of sections, microwave treatment, and 4 M guanidine thiocyanate treatment have been suggested. The objective of our experiment was to obtain the standard pretreatment(s) resulting in optimal immunostaining. In the experiment, successive tissue slides of brain specimens of several Creutzfeldt–Jakob disease and control patients were stained using different combinations of pretreatments.
Antigen Retrieval of Basement Membrane Proteins from Archival Eye Tissues - Antigen retrieval (AR) methods can unmask tissue antigens that have been altered by fixation, processing, storage, or resin interactions. This is particularly important in the study of archival tissues, because primary fixatives and storage times may vary among specimens.
Apotech - Antibodies for apoptosis and inflammation research
Application of Microwave Technology - Application of Microwave Technology to the Processing and Immunolabeling of Plastic-embedded and Cryosections
Applied Immunohistochemistry & Molecular Morphology - Applied Immunohistochemistry & Molecular Morphology provides complete coverage of the diagnostic and prognostic applications of immunohistochemistry (IHC), its contributions to the understanding of the biology of tumors and other lesions, and the technical aspects of setting up a laboratory and interpreting results.
Aptec Diagnostics - Polyclonal antibodies for use in quantitative turbidimetric and nephelometric determinations in human serum
Aquatic Diagnostics - Monoclonal antibodies for aquaculture. Antibodies to detect pathogens in fish and IgM in different fish species.
Araclon Biotech - Makers of 3 polyclonal antibodies: AB1 recognizes both Ab40 and Ab42, AB2 recognizes specifically Ab40, and AB3 recognizes specifically Ab42. They also make an ELISA kit to detect beta-amyloid protein in blood.
Argene Biosoft - Argene: development and production of diagnostic and/or research use reagents in the microbiology field by using immunology or molecular biology methods
Arista Biologicals - Monoclonal and polyclonal antibodies, and colloidal gold conjugates for fertility, infectious diseases, drugs of abuse, etc., as well as antigens for rapid diagnostic tests.
Aurion - AURION is a research oriented company dedicated to and specializing in the development and production of Ultra Small ImmunoGold Reagents, Silver Enhancement Reagents, Blocking Reagents, Incubation Solutions and Conventional ImmunoGold Reagents
Aves Labs - Specializing in chicken antibodies. They sell blocking reagents (BlokHen®) and reagents designed for immunoprecipitation (PrecipHen®) of chicken antibodies; a variety of enzyme- and fluorescence-tagged anti-chicken antibody reagents; and chicken antibodies against epitope tags such as c-myc, hemagglutinin, and FLAG.
Aviva Systems Biology - Aviva Systems Biology (ASB) develops, manufactures and markets reagent tools for gene transcription regulation research. We are best known for high quality novel antibodies, Innovative innovative Gene Regulatory Elements mapping and Analysis (GREMA) Technology and reliable custom gene transcription regulation mapping services
B-Catenin - Identification of B-Catenin in Formalin-Fixed, Paraffin Embedded Rodent Tissue
Bachem - Offers a comprehensive selection of sevices to meet your needs in immunochemicals of your choice.
BD Bioscience Clontech - Provider of GFP, GAL4, cell cycle, phosphoamino acid, phosphokeratin, and other antibodies, as well as many other innovative molecular and cell biology-related products
Beckman Coulter - Monoclonal and polyclonal antibodies to human and animal antigens.
Beecher Instruments - Beecher develops microarray technologies that facilitate high-throughput genomic and proteomic research. Offers automated and manual tissue microarray instruments and accessory tissue arrayer products.
Beta Polymerase - Identification of Beta Polymerase by an 8K Domain Antibody in Paraffin Embedded Rodent Tissue
Bethyl Laboratories - Primary and secondary antibodies to human, rodent and domestic animal serum proteins, affinity purified and conjugated, epitope tags, ELISA kits
Biocare Medical - BIOCARE is a unique manufacturer and supplier of immunostaining systems, histopathology products, monoclonal and polyclonal antibodies and automated staining equipment.
BioCore - BioCore supplies over 10,000 research related products including Antibodies, Immunoassay Kits, Enzymes, Immunotoxins, Reagents, and much more.
Biogenesis - Operating from the South Coast of England and New Hampshire in the US we are able to offer a wide range of Custom Services, in addition to our comprehensive catalogue of over 6,000 products.
BioGenex - BioGenex offers a broad range of reagents, detection kits, antibodies, probes, special stains, tissue microarrays, automated staining and imaging systems to streamline and standardize cellular and molecular analysis.
Biognostik - Supplier of custom-designed, high-quality ANTISENSE sequences and HybriProbes™ for in situ hybridization.
BioLegend - We offer products covering the areas of cell immunophenotyping, cytokines and chemokines, adhesion, phosphorylation, cancer research, cell-cycle analysis, and apoptosis. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications including flow cytometry, ELISA, immunoprecipitation, Western blotting, immunofluorescence microscopy, immunohistochemistry, and in vitro / in vivo functional assays.
BioLink International - Provide Recombinant HIV, HCV and Syphilis antigens for purpose of research and manufacturing. We also provide laboratory consumables to distributors, hospitals and research communities.
Biomarket - Biomarket offers more than 100 products in the field of infectious diseases, monoclonal antibodies, antigens, extracellular matrix, integrin and molecular biology products including modifying enzymes and PCR reagents.
BioMed Central - BioMed Central. Over 100 Open Access journals covering all areas of Biology and Medicine
Biomeda - Full range of detection kits, a diversified line of monoclonal and polyclonal antibodies,
Biomedica - Supplier of endothelin and big endothelin antibodies.
Biomedical Technologies - BTI prepares over 35 different antibodies. Antibodies to the cytoskeletal proteins, growth & attachment factors, lipoproteins, hormones and keratinocyte differentiation markers are also offered. All our products are produced in the USA. Labeled second antibodies and precipitating antibodies are available in convenient packaging.
BIOMOL International - BIOMOL offers a range of biochemicals with applications in signal transduction, immunohistochemistry, gene regulation, apoptosis, neuroscience and drug discovery. In addition to purified enzymes, antibodies, and assay kits, BIOMOL’s innovative products include Nupherin-neuron transfection reagent, Fluor de Lys HDAC assay kit, and Screen-Well compound libraries. www.biomol.com 800-942-0430
Biomol International - The BIOMOL International worldwide network offers specialised reagents for signal transduction research (including antibodies, peptides, enzymes, enzyme activators and inhibitors, second messengers, and assay kits as well as a comprehensive offering of bioactive lipids and neuropharmacologics) and a range of compound libraries for screening (previously available from BIOMOL Research Laboratories) with the innovative products and services for cell biological research available from AFFINITI. This includes a wide range of products for neuroscience research and the world's most comprehensive range of substrates, inhibitors, enzymes, proteins and antibodies for use in ubiquitin, proteasome and COP9-signalosome research. AFFINITI staff also specialise in the manufacture of high quality synthetic peptides and in the synthetic peptide approach to protein-reactive antibody production.
BioProcessing - Antibodies to Ca15-3, Ca27.29, breast tumor antigens, Ca125, ovarian tumor antigen, Ca19-9, GI tumor antigen, CEA, carcinoembryonic antigen, AFP, alpha fetoprotein, Ca72.4, Tag-72, NSE, neuron specific enolase, Cyfra21-1, cytokeratin-19, PSA, prostate specific antigen using cell culture technologies.
Biosense Laboratories - Producers of antibodies and ELISA kits for the detection of biomarker proteins in fish. Products include monoclonal and polyclonal antibodies for the detection of responses to oil hydrocarbons, PAHs, PCBs, dioxins, endocrine disruptors, etc...
Biosonda - Featuring anti-human acrosin and anti-gizzerosine antibodies and Blue Carrier, a highly soluble and purified carrier protein from the hemocyanin family
Biosource - Biosource International, Inc. is a broad based life sciences company focused on providing solutions in the areas of functional proteomics and advanced drug discovery through the development, manufacturing, marketing, and distribution of unique biologically active reagent systems which facilitate, enable, and accelerate pharmaceutical development as well as biomedical research.
BioSource International - Develops, manufactures, markets and distributes monoclonal and polyclonal antibodies, recombinant proteins, custom oligonucleotides and quantitative cDNA amplification kits for the study of immunology and molecular biology
BiosPacific - Antibodies and antigens for the development of diagnostic assays
Biostride - East Coast Biologics - Monoclonal and polyclonal antibodies to drugs of abuse, environmental compounds, hormones and steroids, tumor markers, infectious diseases, therapeutic drugs, and other miscellaneous compounds
Biotinylation of Antibodies - Biotin is used in two-step detection systems in concert with conjugated avidin. Biotin is typically conjugated to proteins via primary amines (i.e., lysines). Usually, between 3 and 6 biotin molecules are conjugated to each antibody.
Biotrend - BIOTREND is specialised in Immunology, Neuroscience, Pharmacology and Radiochemistry, supplying biomedical and pharmaceutical research with a large range of antibodies, radiolabelled products, custom synthesis and antibody production services.
BioVendor Laboratory Medicine - Specializes in the production and distribution of in vitro research products for molecular and cellular laboratory medicine
BMA Biomedicals - Monoclonal antibodies, polyclonal antibodies, and ELISA kits for macrophage and inflammation typing in mouse, human and rats
Boston Biochem - Antibodies and assay kits for the ubiquitin proteasome pathway
BRDU Mouse - Detection of BRDU in formalin-fixed, paraffin-embedded mouse tissue
BRDU Rat - Detection of BRDU in formalin-fixed, paraffin-embedded rat tissue
Calbiochem - Distributor of an extensive range of monoclonal and polyclonal antibodies, secondary antibodies and their conjugates, plus many accessory products for immunoassay and immunoseparation.
CanAg Diagnostics - A biotechnology company specialising in the development of specific reagents (monoclonal antibodies) and laboratory tests that can be used for sensitive and specific determination of tumor markers and biochemical markers of brain disease
Capricorn - Manufacturer and distributor of immunodiagnostic products including monoclonal and polyclonal antibodies to infectious diseases, hormones. steroids, tumor markers, and serum proteins.
Cayman Chemical - Cayman Chemical Company is helping make research possible by supplying scientists worldwide with biochemical tools in research disciplines such as cancer, nitric oxide, neuroscience, apoptosis, oxidative injury, endocrinology, and much more. We specialize in assay kits for the measurement of eicosanoids, free radical biomarkers, cyclic nucleotides, cytokines, hormones and nitric oxide. In addition, Cayman offers a broad range of quality biochemicals including eicosanoids, nitric oxide reagents, and a variety of related lipids, fatty acids, enzymes, and antibodies.
CCK-A Receptor - Detection of CCK-A Receptor (G-17) in Formalin-Fixed, Paraffin-Embedded Rat Tissue
CD10 - CD10 clone 56C6 Protocol for DakoCytomation Autostainer. NordiQC
CD10 - CD10 clone 56C6 Protocol for DakoCytomation Autostainer. NordiQC
CD10 is expressed in a subset of chromophobe renal cell carcinomas - CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas.
CD117 - CD117 (polyclonal) Staining Protocol for Autostainer
CD117 - CD117 (polyclonal) Staining Protocol for Ventana BenchMark XT
CD20 - CD20 clone L26 Protocol for DakoCytomation Horizon. NordiQC
CD20 Expression in Hodgkin and Reed-Sternberg Cells of Classical Hodgkin’s Disease - CD20 can be expressed in Hodgkin and Reed-Sternberg (HRS) cells of classical Hodgkin’s disease (HD), but its clinical significance remains controversial. Therefore, we correlated CD20 expression with presenting features and clinical outcome of untreated patients with classical HD.
CD23 - CD23 clone 1B12 Protocol for DakoCytomation Autostainer. NordiQC
CD23 - CD23 clone 1B12 Protocol for DakoCytomation Autostainer. NordiQC
CD23 - CD23 clone 1B12 Protocol for Ventana NexES. NordiQC
CD23 - CD23 clone MHM6 Protocol for DakoCytomation Autostainer. NordiQC
CD3 - CD3 clone PS1 Protocol for DakoCytomation Autostainer. NordiQC
CD3 - CD3 clone F7.2.38 Staining Protocol for DakoCytomation Autostainer.
CD3 - CD3 clone PS1 Staining Protocol for Ventana Benchmark.
CD3 - CD3 clone PS1 Staining Protocol for DakoCytomation Autostainer