Immunohistochemistry - In Situ Hybridization

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  • Immunohistochemistry - In Situ Hybridization Images:

  • Immunohistochemistry Image Gallery

  • Immunofluorescence Image Gallery

  • In Situ Hybridization Image Gallery

  • Immunohistochemistry - In Situ Hybridization Staining Protocols:

  • ABC Protocol for Monoclonal Antibodies

  • ABC Protocol for Polyclonal Antibodies

  • ABC Protocol Monoclonal Antibodies (Frozen Sections)

  • ABC Protocol Polyclonal Antibodies (Frosen Sections)

  • ABC Px DAB for FFPWs for all species of Primary Abs

  • Actin (Muscle Actin) Protocol for ES/NexES

  • Actin (Smooth Muscle) Protocol for Autostainer (Cytospins)

  • Actin alpha. Muscle - specific

  • Adrenocorticotropin (ACTH) Protocol for ES/NexES

  • ALK Protein Protocol for ES/NexES

  • Alpha-1-Antitrypsin Protocol for ES/NexES

  • Alpha-Sarcoglycan (Adhalin) Protocol for NexES/ES

  • Alpha-Synuclein Protocol for ES/NexES

  • Amyloid A Component Protocol for ES/NexES

  • Antigen Retrieval Tris pH 10 using M/waveable pressure cooker

  • APAAP General Protocol (Mouse Primary)

  • APAAP General Protocol (Rabbit Primary)

  • Apolipoprotein D Protocol for ES/NexES

  • Aspergillus Protocol for ES/NexES

  • B-catenin staining FFPET

  • Bcl-2 Oncoprotein Protocol for ES/NexES

  • Bcl-6

  • Bcl-6 Protocol for ES/NexES

  • BCL6 on FFPET

  • Beta Tubulin Monoclonal in FFPW tissues 1

  • Beta tubulin Polyclonal in FFPWs 2

  • Beta-Dystroglycan Protocol for NexES/ES

  • Beta-Sarcoglycan Protocol for NexES/ES

  • Blocking solution for non-specific binding in pwax sections

  • Bob-1 Protocol for ES/NexES

  • BrdU

  • BrdU Ag retrieval Pwax sections

  • BRDU in FFPE Mouse Tissue

  • BrdU in FFPWs Rat,mouse, chick, human

  • C4d Complement

  • Calbindin D detection stABC pxDAB

  • Calcitonin Protocol for ES/NexES

  • Calretinin Protocol for Autostainer

  • Calretinin Protocol for Autostainer (Cytospins)

  • Candida Albicans Protocol for ES/NexES

  • Carcinoembryonic Antigen (CEA) Protocol for ES/NexES

  • CD10 on FFPET

  • CD10 Protocol for Autostainer

  • CD10 Protocol for ES/NexES

  • CD117 (c-kit) Protocol for ES/NexES

  • CD133 localisation in FFPWs of rat adult brain

  • CD138 on FFPET

  • CD138 Protocol for ES/NexES

  • CD14

  • CD15 Protocol for ES/NexES

  • CD1a Protocol for ES/NexES

  • CD20 Protocol for ES/NexES

  • CD21 Protocol for ES/NexES

  • CD22 Protocol for ES/NexES

  • CD23 Protocol for ES/NexES

  • CD3 Polyclonal

  • CD3 Protocol for ES/NexES

  • CD3 Rabbit monoclonal in mouse FFPW section

  • CD3 Rabbit polyclonal on mouse FFPW sections

  • CD30 Protocol for ES/NexES

  • CD31 Protocol for ES/NexES

  • CD34

  • CD34 Protocol for ES/NexES

  • CD4 Protocol for ES/NexES

  • CD40 Protocol for ES/NexES

  • CD42b Protocol for ES/NexES

  • CD43 Protocol for ES/NexES

  • CD45T0 Protocol for ES/NexES

  • CD5 Protocol for ES/NexES

  • CD56 (NCAM) Protocol for ES/NexES

  • CD56 ERIC NCAM on FFPWs

  • CD56 H28 NCAM on FFPW sections of mouse

  • CD57 localisation in FFPWs of mouse, rat and chick sections

  • CD57 Protocol for ES/NexES

  • CD68 Protocol for ES/NexES

  • CD79a Protocol for ES/NexES

  • CD8 Protocol for ES/NexES

  • CD99 Protocol for ES/NexES

  • Chromogranin A Protocol for ES/NexES

  • Cyclin D1

  • Cyclin D1 Protocol for ES/NexES

  • Cytokeratin 19 Protocol for ES/NexES

  • Cytokeratin High Molecular Weight Protocol for ES/NexES

  • Cytokeratin MNF116 Protocol for ES/NexES

  • Cytokeratin Pan- Protocol for Autostainer (Cytospins)

  • Cytomegalovirus (CMV) Protocol for ES/NexES

  • DAB Stock solution

  • Delta-Sarcoglycan Protocol for NexES/ES

  • Desmin Protocol for Autostainer (Cytospins)

  • Desmin Protocol for ES/NexES

  • Dewaxing slides for immunostaining

  • Drosophila embryo sections for BP102

  • Drosophila menanogaster Processing for Immunostaining ( Fruit fly embryos)

  • Dysferlin Protocol for NexES/ES

  • Dystrophin (c-terminus) Protocol for NexES/ES

  • Dystrophin (n-terminus) Protocol for NexES/ES

  • Dystrophin (rod domain) Protocol for NexES/ES

  • Emerin Protocol for NexES/ES

  • Epithelial Antigen (Ber-EP4) Protocol for ES/NexES

  • Epithelial Membrane Antigen (EMA) Protocol for ES/NexES

  • Epstein-Barr Virus (EBNA2) Protocol for ES/NexES

  • Epstein-Barr virus (Latent Membrane Protein) Protocol for ES/NexES

  • Estrogen Receptor (cytospin) Protocol for Autostainer

  • Estrogen Receptor (ER) Protocol for ES/NexES

  • Estrogen Receptor (Smears and Cytospins) Protocol for ES/NexES

  • Fasciclin II in Fruit fly FFPWs

  • Fixing GFP expressing tissue

  • Gamma-Sarcoglycan Protocol for NexES/ES

  • GAP43 Immunostaining in FFPWs

  • GAP43 phosphospecific inFFPWs Mouse, rat and chick

  • Gastrin Protocol for ES/NexES

  • GFAP for Astrocytes FFPWS

  • GFAP Protocol for Autostainer (Cytospins)

  • GFP localisation in FFPW sections

  • Glial Fibrillary Acidic Protein (GFAP) Protocol for ES/NexES

  • Glucagon Protocol for ES/NexES

  • Glycophorin A Protocol for ES/NexES

  • Granzyme B Protocol for ES/NexES (1)

  • Granzyme B Protocol for ES/NexES (2)

  • Heat Induced Epitope Retrieval (HIER) Microwave Method

  • Heat Induced Epitope Retrieval (HIER) Pressure Cooker

  • Helicobacter Pylori Protocol for ES/NexES

  • Hepatitis B Core Antigen (HBcAg) Protocol for ES/NexES

  • Hepatitis B Surface Antigen (HBsAg) Protocol for ES/NexES

  • Hepatocyte Protocol for ES/NexES

  • HIER M/W pressure- cooker with TRIS 10mM pH10

  • Histone H3 ( phospho) Mitosis marker FFPWs

  • HLA-DR Alpha Chain Protocol for ES/NexES

  • HMB45 Protocol for ES/NexES

  • HuC/D antigens demonstration in FFPWs

  • Human Chorionic Gonadotropin (hCG) Protocol for Autostainer

  • Human Chorionic Gonadotropin (hCG) Protocol for ES/NexES

  • Human Growth Hormone (hGH) Protocol for ES/NexES

  • Human Herpesvirus (type 8) Protocol for ES/NexES

  • Immuno Gold Silver (IGS)

  • Immuno Gold Silver Staining (with Colloidal Gold)

  • Immunofluorescence Labeling of Cells

  • Immunoglobulin A (IgA) Protocol for ES/NexES

  • Immunoglobulin G (IgG) Protocol for ES/NexES

  • Immunoglobulin M (IgM) Protocol for ES/NexES

  • Indirect Immunoenzyme Technique

  • Ki67 (cytospin) Protocol for Autostainer

  • Ki67 (Smears and Cytospins) Protocol for ES/NexES

  • Ki67 for FFPWs

  • Ki67 Protocol for ES/NexES

  • Labeled Streptavidin Biotin (LSAB) General Method

  • Lamin A&C Protocol for NexES/ES

  • Laminin detection in FFPWs

  • LCA Protocol for ES/NexES

  • Lysozyme (Muramidase) Protocol for ES/NexES

  • M. Tuberculosis on FFPET

  • Mast Cell Tryptase (MCT) Protocol for ES/NexES

  • MCAD localisation in FFPWs

  • Melan-A Protocol for ES/NexES

  • Melanoma Cocktail Rapid immunostaining protocol

  • Melanoma Marker (Pan) Protocol for ES/NexES

  • Merosin Laminin Alpha 2 Chain Protocol for NexES/ES

  • Merosin M-chain Protocol for NexES/ES

  • Mesothelial Cell (HBME-1) Protocol for ES/NexES

  • Microtubule-associated Protein, Neurone-specific in FFPWs

  • MLH-1 on FFPET

  • Mowiol Mountant Firm set Immunofluorescence

  • MSH-2 on FFPET

  • Myogenin in FFPWs

  • Myosin heavy chain typeII ( fast) in FFPWs

  • Nestin localisation in FFPWs

  • Neurofilament 160 ( RMO ) in FFPWs

  • Neurofilament 160 (NN18) in FFPWs

  • Neurofilament associated protein

  • Neurofilament Protein Protocol for ES/NexES

  • Neuron Specific Enolase (NSE)

  • Neuronal Nuclei (NeuN) Protocol for ES/NexES

  • Neurotactin in fly embryo FFPWs

  • Oct-2 Protocol for ES/NexES

  • p16 INK4a Protocol for ES/NexES

  • p21 WAF1/CIP1 in FFPWs of developing mouse muscle

  • p53 (cytospins) Protocol for Autostainer

  • p53 Protocol for ES/NexES

  • PAP General Protocol (Mouse Primary)

  • Parvovirus B19 Protocol for ES/NexES

  • Pax-5 Protocol for ES/NexES

  • PGP 9.5 in FFPWs

  • Placental Alkaline Phosphatase (PLAP) Protocol for ES/NexES

  • Pneumocystis carinii Protocol for ES/NexES

  • Progesterone Receptor (Cytospins) Protocol for Autostainer

  • Progesterone Receptor (PGR) Protocol for ES/NexES

  • Progesterone Receptor (Smears and Cytospins) Protocol for ES/NexES

  • Prolactin

  • Prostate Specific Antigen (PSA) Protocol for ES/NexES

  • Proteinase K Digestion

  • PU.1 Protocol for ES/NexES

  • Renal cell Carcinoma Marker on FFPET

  • Resin sectioning of wholemount in situ/immuno labelled specimens

  • Respiratory Syncytial Virus (RSV) Protocol for ES/NexES

  • S 100 Protocol for ES/NexES

  • Semi-permanent mounting medium for immunofluorescence

  • Serotonin

  • Somatostatin Protocol for ES/NexES

  • Spectrin Protocol for NexES/ES

  • StABC (Streptavidin Biotin Complex Method)

  • streptABC Peroxidase Dako kit/DAB method for FFPWs for all species of primary Antibody

  • Streptavidin-Nanogold-Silver-Staining

  • SV40 T-Ag (Ab-1) Protocol for ES/NexES

  • SV40 T-Ag (Ab-2) Protocol for ES/NexES

  • SV40 T-Ag Ab-2 (Cytospins) Protocol for ES/NexES

  • Synaptophysin Protocol for ES/NexES

  • Terminal Deoxynucleotidyl Transferase (TdT) Protocol for ES/NexES

  • Toxoplasma Gondii Protocol for ES/NexES

  • Trk (C-14) on pwax mouse embyro section

  • Trypsin Digestion

  • Trypsin Treatment

  • TTF-1 Protocol for Autostainer (Cytospins)

  • Tyramide amplification ABC- DAB - Px method

  • Tyrosine hydroxylase in HIER FFPWs mouse

  • ubiquitin on FFPET

  • VGLUT1 on human rat mouse pwax sections

  • Vimentin Protocol for Autostainer (Cytospins)

  • Vimentin Protocol for ES/NexES

  • Vimentin V9 on rat pwax sections

  • Von Willebrand Factor (Factor VIII) Protocol for ES/NexES


  • Immunohistochemistry - In Situ Hybridization News:

  • 225,000 PRIMARY ANTIBODIES and Where to Get Them

  • A Simple, Reliable, and Sensitive Method for Multiple Immunoenzyme Staining

  • A Universal Antigen Retrieval Method

  • Abbott and BioGenex Enter Agreement for Automated Molecular Diagnostics System

  • ALCAM/CD166 in in breast cancer

  • Anaplastic Lymphoma Kinase (ALK)

  • Apoptosis, Cell Death and Cell Proliferation

  • Application of microarray technology in pulmonary diseases

  • Applied Immunohistochemistry & Molecular Morphology

  • Autofluorescence Eliminator Reagent

  • BCL-2

  • Biotinyl-tyramide-based In Situ Hybridization

  • Breast Carcinoma vs. Pulmonary Adenocarcinoma

  • Calretinin

  • CD10 (CALLA) Immunohistochemistry

  • CD155/PVR

  • CD56 (NCAM)

  • cDNA microarrays

  • CDX2

  • CDX2

  • Chromogenic in situ hybridization (CISH)

  • Claudin 7

  • Claudin-1, -3 and -4 proteins and mRNA expression

  • Coexistence of HER2 over-expression and p53 protein

  • Collecting duct carcinoma of the kidney (CDC)

  • Cutaneous Basal Cell Carcinoma and Squamous Cell Carcinoma

  • Cyclin A and cyclin D1

  • Cytokeratin 5/6

  • Cytokeratin AE1/AE3

  • Cytopathology - from an Immunohistochemist’s Perspective

  • D2-40

  • Dako goes Rabbit

  • DAX-1 expression in human breast cancer

  • Desmoplastic small round cell tumor

  • Differential Diagnosis of Cytokeratin “Dots”

  • Double-label Fluorescent Immunohistochemistry

  • Dual Fluorescent In Situ Hybridization and Immunohistochemical Detection

  • Endocervical vs. Endometrial Adenocarcinoma

  • Endogenous Biotin Artifact

  • Enhanced diagnostic immunofluorescence using biopsies transported in saline

  • Enrichment methods to detect bone marrow micrometastases

  • Epidermal Growth Factor Receptor and Erbitux

  • Evaluation of Follicular or Nodular Lymphoid Lesions

  • Evaluation of Hydropic Placentas (Hydropic Degeneration vs. Partial Mole vs. Com

  • Expression of pS2 in prostate cancer

  • FISH Solution for Lymphoma

  • HER-2/neu and c-Kit (CD117) in malignant melanoma

  • High Molecular Weight Cytokeratin (34βE12)

  • Histologic Spring 2004

  • hMLH1 and hMSH2

  • Human CD57+ germinal center-T cells

  • Human Herpesvirus Type 8 (HHV8)

  • ImmPRESS™ - New Staining System from Vector Laboratories

  • Immunohistochemical markers of prostate carcinoma

  • Immunohistochemistry

  • Immunohistochemistry in Amyloidosis

  • Immunohistochemistry in Clear Cell Carcinomas

  • Immunohistochemistry in DFSP vs. DF-FH

  • Immunohistochemistry in Lymph Node Histiocytosis

  • Immunohistochemistry in the Differential Diagnosis of Schwannoma and Neurofibrom

  • Immunohistochemistry in the Evaluation of Atypical Epithelial Cells in Effusions

  • Immunohistochemistry in thyroid carcinoma

  • Immunohistochemistry in Urothelial Dysplasia

  • Immunohistochemistry Staining Protocols

  • Immunohistochemistry Staining Protocols

  • Immunohistochemistry: New Visualization System

  • Immunostaining of imprint smears with the 'MCW melanoma cocktail'

  • Immunostains for b-catenin in Diagnostic Pathology

  • In Situ Hybridization

  • In Situ Hybridization with Nanogold-Streptavidin

  • Interleukin-2 and its receptor complex (a, ß and γ chains)

  • KATP channel subunits

  • Ki-67 clone K-2: A useful marker of fat cells and lipoblasts

  • MCM2

  • Metallothionein expression in renal cell carcinoma

  • Microarray analysis in clinical oncology

  • MMP-13 and TIMP-1 in head and neck squamous cell carcinomas

  • Multicolor-FICTION

  • New Instrument for Cancer Diagnostics

  • New Web site!

  • Oct3/4 A New Marker of Embryonal Carcinoma and Seminoma

  • Oncotech Offers New Genomic Test for Lung Cancer

  • ONE-STEP RNA probe synthesis templates

  • Optimal Titering of Primary Antibodies

  • p14ARF expression in invasive breast cancers and ductal carcinoma in

  • p16INK4a

  • p21WAF1/CIP1 and HER-2/ neu

  • p27Kip1

  • P504S (a-Methylacyl-CoA-Racemase)

  • p63

  • p63/P504S Antibody Cocktail in Prostate Biopsies

  • Perivascular Epithelioid Cell Tumores

  • Platelet derived growth factor receptor alpha (PDGFR-α)

  • Polysomy of chromosome 17 and overexpression of ERBB2

  • Prostate stem cell antigen (PSCA)

  • Protein p16

  • Recognition of Early Myocardial Infarction

  • Respiratory Syncytial Virus (RSV) Immunohistochemistry

  • S100A7 (psoriasin) and Jab1 in ductal carcinoma in situ of the breast

  • Simultaneous Detection of EGFP and Cell Surface Markers by Fluorescence

  • TFE3 Immunostains in the Diagnosis of Alveolar Soft Part Sarcoma and Other Tumor

  • The Antigen–Antibody Reaction in Immunohistochemistry

  • The utility of cytokeratins 7 and 20 (CK7/20) immunohistochemistry

  • Tissue Micro Array (TMA)

  • Tissue Protection Immunohistochemistry

  • Traps in Sentinel Lymph Node Biopsies

  • True Positive vs. False Positive Staining

  • Ubiquitin Immunohistochemistry in Alzheimer’s Disease

  • Ultrarapid Ki-67 immunostaining in frozen section interpretation of gliomas

  • UroVysion FISH

  • Utility of Ki-67 (MIB-1) Immunostaining in Cervical Biopsies

  • Utility of p16 (INK4a) Immunostaining in Cervical Biopsies

  • Vascular endothelial growth factor (VEGF)-C

  • Villin Immunohistochemistry

  • Wilms Tumor Gene Protein (WT1)

  • XHM

  • Xmatrx(TM)

  • ZAP-70 in Chronic Lymphocytic Leukemia

  • “Basal-like” or “Basaloid” Breast Carcinoma


  • Immunohistochemistry - In Situ Hybridization Forum Categories:

  • Immunohistochemistry

  • In Situ Hybridization

  • Welcome


  • Immunohistochemistry - In Situ Hybridization Forums:

  • Automated Immunohistochemistry - Here you can discuss anything related to Automated Immunohistochemistry.

  • Automated In Situ Hybridization - Here you can discuss anything related to Automated ISH and FISH.

  • General Immunohistochemistry - This forum is open for all to discuss anything related to Immunohistochemistry and Immunofluorescence techniques.

  • General In Situ Hybridization - This forum is open for all to discuss anything related to In Situ Hybridization (ISH) and Fluorescent In Situ Hybridization (FISH) techniques.


  • Immunohistochemistry - In Situ Hybridization Topics / Posts:

  • about storage of embryos

  • About the TSA system (Perkin)

  • Abs towards human HLA class I antigens

  • adressing the sense probe signal problem

  • AEC a better substrate in IHC

  • alternative mounting solution (to DEPEX)

  • Alu probe

  • amti rat MMP12 or MMP14

  • anti Cathepsin S on rat

  • anti Cy5

  • anti integrin alpha v beta 3 on mouse tissue

  • Anti TNFa mouse tissues

  • anti-Tet-repressor antibody

  • anti-VP16 antibody

  • antibodies against C5b-9 Complement Terminal Complex

  • Antibody Diluents, Help!

  • Antibody penetration issues

  • antibody VEGF

  • antigen recognition

  • Antigen retreavel

  • Any protocols for removing lipids in sections prior to ISH?

  • aquaporins

  • Artifacts

  • Aspergillus

  • autofluorescence

  • Autofluorescence in rat brain tissue after lesions

  • automated ISH

  • Background problems

  • background problems with isotope probes

  • Background Staining on frozen embryo liver

  • background staining with hrp detection

  • Background staining.

  • Base mismatch

  • basic equipment(s) for ISH

  • Basic Questions

  • Basic theory of ISH

  • Best mounting media?

  • Best way finding antibodies

  • bibliography about probe design

  • biotin blocking kits

  • Blimp-1

  • blood vessels in brain

  • bone problems

  • Books for ISH

  • Botulinum Toxin A (BOTOX) IHC?

  • Bouin fixation with ISH

  • BrdU double labelling

  • Brdu in vivo @ in vitro

  • Bubbles on the brain sections

  • c-Kit antibody

  • can BSA increase background???

  • Can you do FISH on live bacterial cells?

  • CD3

  • CD38 Formalin Fixed Tissue

  • CD45 antigen not just in leukocytes??

  • Cells appear brown in BF microscopy in abscence of DAB

  • checking RNA -probes

  • Citraconic Anhydride

  • Citraconic anhydride recipe

  • Citraconic revisited

  • Colormetric detection and quantification

  • Combination ISH/Immunofluorescence

  • Commercial source for gene probes - recommendations?

  • Compatibility of non-perfused tissue and Immuno...

  • control probe

  • Control probe for Aedes mosquitoes or Drosophila

  • counterstain nucleus

  • Counterstaining of DIG-labelled riboprobes

  • course

  • Cross-Hybridization

  • Crystalline silica induced inflammation

  • Cyclin D2 detection

  • Cytokeratin stains

  • DAB Away Recipe

  • DakoCytomation GenPoint TSA System!!!

  • DAPI

  • DC-SIGN

  • Debris after development

  • decalcification

  • Decalcification

  • decalcification

  • detect nuclear mRNA

  • DIG control riboprobes

  • Dig-RNA probes

  • Disposal of used DAB solution

  • dispose PF

  • DNAse to reduce sense probe staining

  • double immunofluorescence protocol about bone

  • Double Immunofluorescence with two mouse mAb

  • Double ISH

  • Double ISH problem

  • Double stain, incompatible pretreatments

  • Draq5

  • Endogenous Alkaline Phosphatase

  • Factor XIIIa

  • Feulgen kit?

  • few questions about ish?

  • FISH

  • FISH in embryos or spermatozoa

  • Fixation and postfixation

  • Frozen brain.

  • fuzzy signal

  • General in situ protocol

  • general info on ISH

  • GFP fluorescence in mouse brain tissue

  • glutamatergic cell body marker

  • Gold medal for Ole.........

  • good anti-Biotin antibody?

  • Happy, belated, birthday Hogne!

  • HEEEELLLLPPP!

  • HELP with FISH

  • help! anyone has experience of caveolin immunostaining

  • Hi Ventana users

  • High background in ovaries

  • how to design RNA probe

  • how to reduce background staining?

  • hyaluronic acid detection

  • hybridization buffer

  • Hybridization buffer recipes...

  • hydrolysis of probe

  • I just ordered some new Abs...need user comments, please

  • IF nuclear stain other than DAPI

  • IgG biotynilation

  • IHC of cytoplasmic antigen as readout for RNAi

  • IHC problem! everything turning brown, even negative control

  • Immuno in frozen sections

  • immunofluorescence

  • Immunofluorescence AND ISH on mouse tissue

  • Immunohistochem apoptotic markers

  • Immunohistochemistry Protocols

  • immunohistochemistry vs. immunofluorensce

  • immunostaining H&E

  • Impressed?

  • In situ and immuno together

  • In situ hybridization on culture cells

  • In situ PCR

  • in situ RT-PCR - fixatives

  • Interleukin 6

  • interleukins staining IHC

  • ISH background in glass

  • ISH for free floating sections and cryoprotection

  • ISH for IFNgamma mRNA

  • ISH for new a gene

  • ISH in Bone

  • ISH on bovine prions

  • ISH on Cryosections

  • ISH on embryo cryosection

  • ISH on plants tissue

  • just the biochemicals do

  • just the biochemicals do

  • Ki-67 Quantitation in Mouse Bronchioles

  • LacZ activity and ISH

  • LacZ staining hard tissue

  • large amount of osteopontin background staining

  • Lectin PNA conjugates

  • Localisation of mRNA

  • Localization in Plant Tissue using ISH

  • Loss of signal in DIG in-situs

  • Low signal with DRD2 radioactive

  • Low signal with Isotopic In situ hybridization

  • Making anitsense riboprobes

  • Mallory-staining

  • mast cells in mice

  • MCM2

  • Melanin pigment removal

  • Mitochondrial Probe problems

  • MLH-1

  • Most appropriate protein blocking solution ?

  • mouse probe

  • mRNA degradation in frozen tissue

  • mRNA degradation in frozen tissue

  • multi probes

  • Need contract IHC for panel of human tissue

  • Need some antibodies

  • new guy

  • New to IF! Please review my protocol!

  • New to IHC - startup equipment?

  • newbie Q: negative control is stained

  • Nitrotyrosine Staining

  • no signal in in situ using b-actin

  • non-specific binding

  • OCT compound

  • Ole's New HRP kit

  • oligo dT hybridization to mRNA in stress granules

  • Omiting Normal Serum in blocking lessen background?

  • optimum amount of probe-please help!

  • Organ Culture & BrdU

  • P53 immunistainning

  • Paul - a question of sense and nuclear negative controls

  • PCNA brown background

  • PCNA brown background

  • PCR DIG Probe Kit for RNA detection?

  • per1 in hamster brains

  • perfusion of liver tissue

  • Phosphoantibodies

  • pink background with DIG staining

  • Please Help; Non-specific stainning everywhere

  • Poly d(T) probe

  • positive blank sections

  • Positive sense probe

  • Positive signal in sense probe

  • probe design and label for FISH

  • Problem with erys

  • Problem with Fluorojade-B

  • problem:precipitate/debris with any chromogen

  • problems with bone tissue

  • Problems with DAPI

  • problems with sectioning.

  • Problems with Staining

  • Problems with the background...IHC in chick embryos

  • Proteinase k alternative for ISH/IHC

  • protocol ER beta

  • Protocol for ER, PR and Her-2

  • protocols

  • radioactive in-situ

  • radioactive in-situ hybridization

  • Radioactive Labeling of Probe

  • rat-specific nuclear stain

  • Resources to locate those proficient in immunohistochemistry

  • Retina IF

  • Retriever EMS

  • Revised Mowiol formulation in "Protocols"

  • rhodamine at 37 degrees

  • Riboprobe without cloning for ISH

  • Riboprobes or Oligoprobes..?

  • RNALater

  • Search for canine antibodies of TNF, IFN, IL

  • sections ad slides

  • Sensitive Dig in situ protocol

  • Sensitive TSA ISH combined with IHC

  • Sensitivity X-ray film vs liquid emulsion

  • Signal amplification

  • signal in nucleaus

  • skipping the protease step

  • slide-coating protocol

  • slides for in-situ

  • SlowFade Gold/Prolong Gold vs. Permount

  • software to organise IHC slides

  • Some problem in signal....

  • some very basic questions

  • storage

  • Storage of embryonic and adult tissues for wax ISH

  • Storage of Frozen OCT blocks

  • Storing fixed cells

  • Tissue Preparation for ISH

  • TMA artifact

  • transcription factor in mouse lymphoid tissue sections

  • Trilogy Ag retrieval soln

  • trouble with ISH

  • TUNEL staining

  • Tween and (NH4)2SO4

  • Tween and (NH4)2SO4

  • Tyramide Signal Amplification and blurry DAPI

  • Urovysion-SpectrumGold Problems

  • use of citrate buffer

  • Use of TSA kits

  • Very Basic Question

  • Very useful form for beginers

  • Washed tissue?

  • What concentration to use for anti-DIG-POD Ab?

  • what label?

  • Which is more specific?

  • whole (bacterial) cell FISH

  • Whole mout in situ hybridization using 60mer oligos

  • why bubbles can cause background?

  • WT1 and PTEN

  • Y chromosome

  • y-chromosome specific antibody

  • ZAP-70


  • Immunohistochemistry - In Situ Hybridization Downloads:

  • ABC Technique - PowerPoint presentation of Avidin Biotin Complex (ABC) Technique.

  • Adobe Acrobat Reader - To read some of the files hosted on this web site, you may nead Adobe Acrobat Reader.
    Adobe Reader 6.0 is the newest version of the familiar Adobe Acrobat Reader software. It also replaces Adobe Acrobat eBook Reader, software for viewing high-fidelity eBooks on your notebook or desktop computer.

  • APAAP Technique - PowerPoint presentation of Alkaline Anti-Alkaline Phosphatase (APAAP) Technique.

  • DAKO Handbook - Theory and application of immunlabelling techniques

  • EBER In Situ Hybridization - PowerPoint presentation of Epstein Barr Virus (EBER) In Situ Hybridization.

  • EBER In Situ Hybridization and CD20 Immunohistochemistry - PowerPoint presentation of Epstein Barr Virus (EBER) In Situ Hybridization combined with CD20 Immunohistochemistry

  • EBER In Situ Hybridization and CK Immunohistochemistry - PowerPoint presentation of Epstein Barr Virus (EBER) In Situ Hybridization in combination with Cytokeratin Immunohistochemistry.

  • EBER In Situ Hybridization and Kappa/Lambda Double Immunofluorescence - PowrPoint presentation of Epstein Barr Virus (EBER) In Situ Hybridization combined with Kappa and Lambda Light Chain Double Immunofluorescence

  • HPV In Situ Hybridization and CK Immunohistochemistry - PowerPoint presentation of Human Papilloma Virus (HPV) In Situ Hybridization combined with Cytokeratin Immunofluorescence

  • Immuno Gold Silver Technique - PowerPoint presentation of Immuno Gold Silver (IGS) Technique.

  • Immunohistochemistry vade mecum - An excellent piece of work by Dr. Paul W. Bishop You can search for tumours to find out their pattern of immunoreactivity or look for individual antibodies to check their specification. There are links to all the references through PubMed. This amazing programme is small, easy to install and runs on any PC.
    To download the latest version, go to author's Home Page

  • LSAB Technique - PowerPoint presentation of Labelled Streptavidin Biotin (LSAB) Technique.

  • Non Biotin Amplification - PowerPoint presentation of Non Biotin Amplification using Fluorecein-labelled antibody.

  • PAP Technique - PowerPoint presentation of Peroxidase Anti-Peroxidase (PAP) Technique

  • Polymer Detection - PowerPoint presentation of Polymer Detection.

  • Technical Immunohistochemistry - TECHNICAL IMMUNOHISTOCHEMISTRY: Achieving Reliability and Reproducibility of Immunostains.
    By Rodney T. Miller, M.D. Director of Immunohistochemistry ProPath Laboratory, Inc.


  • Immunohistochemistry - In Situ Hybridization Web Links:

  • 35S-labeled probe - In Situ Hybridization using 35S-labeled probe, Detection by emulsion or film autoradiography. Frozen Sections.

  • A Concise Guide to cDNA Microarray Analysis - Standard protocol including Array Fabrication, Probe Preparation and Hybridization, and Data Collection, Normalization and Analysis. This is a 27-page PDF file. (The Institute for Genomic Research)

  • A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue Sections - A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue Sections

  • A New Rapid Immunohistochemical Staining Technique Using the EnVision Antibody Complex - A New Rapid Immunohistochemical Staining Technique Using the EnVision Antibody Complex

  • A Novel Quality Control Slide for Quantitative Immunohistochemistry Testing - We introduce a novel quality control technology that may improve intra- and interlaboratory immunohistochemistry (IHC) standardization. The technology involves the creation of standardized antibody targets that are attached to the same slides as the patient sample. After IHC staining, the targets turn the same color as the stained cells or tissue elements. Unlike current clinical practice, our proposed targets are neither cells nor tissue sections.

  • Abazyme - A variety of antibodies including anti-human cytokine and anti-human CD antigens antibodies

  • Abcam - Mission:"...to build the largest catalog of the best antibodies in the world"

  • Abgent - Abgent develops innovative reagents and technologies to profile post-translational modifications of the proteome.

  • Abkem Iberia - Polyclonal antibodies for food and environmental contaminants, allergens, and growth promoters

  • Abnova Corporation - Abnova is the world largest monoclonal antibody manufacturer. We have a capacity of generating 500 mouse monoclonal antibodies per month. Rather than the traditional method of monoclonal antibody production, Abnova is taking a genomic/proteomic approach for the antibody development. Our goal is to have at least one antibody to every human expressed gene in human genome. We manufacture all our products, recombinant proteins and antibodies, in house in our stat-of-art facility in Taiwan. In most cases, we have multiple clones for our monoclonal antibodies, and their access is available to our industrial partners and collaborators from the academic institutions.

  • Academy Bio-Medical Co - Specializing in Human Lipoproteins, Apolipoproteins and Polyclonal Antibodies.

  • Accurate Chemical & Scientific - Accurate has specialized in products for basic and R&D research as well as routine laboratory work. Polyclonal and monoclonal antibodies

  • Acris - Where ever your research interests may be and what ever animal model you work with you will have a good chance to find what you need.

  • ACTIgen - Develops and markets rProteinL for detection and purification of antibodies

  • Active Motif - Kits and reagents for monitoring phosphorylation and other events that change the activation state of transcription factors, signalling proteins and receptors.

  • ADI - Custom peptides and antbodies. They also sell a unique collection of antibodies to receptors, transporters, neurobiologicals, and much more.

  • Advanced Biotechnologies Inc - Supplier of monoclonal antibodies to the following viruses: HIV-1, SIV, HSV-1, HSV-2, CMV, EBV, VZV, HHV-6, HHV-7, HHV-8 and polyclonal antibodies to HIV-1, HTLV-1, HHV-8 vIL-6, and SIV

  • Advanced ImmunoChemical Inc. - Immunological Reagents worldwide for diagnostics sector & research needs.

  • Advanced Targeting Systems - An international supplier of neuroendocrine and neuroscience polyclonal and monoclonal antibodies. ATS also provides agents for the targeting and elimination of specific cell types

  • Advances in Cytochemical Methods for Detection of Apoptosis - Key Words: apoptosis, cytochemistry, TUNEL, ISNT, annexin V, caspases, ricin

  • Affibody - Affibody is a biotech company that uses innovative protein-engineering technologies for the development of products for biotherapeutics and biotechnology applications. The Affibody® molecule, which is a key component of the company’s technology, mimics a monoclonal antibody in many ways, and its properties make it a superior choice for many applications.

  • Affinity BioReagents - ABR—Affinity BioReagents, a privately held corporation, offers over 2,600 industry leading research reagents and custom antibody production services to the worldwide research community. The ABR product portfolio includes primary and secondary antibodies, proteins, peptides and viral expression kits supporting 34 distinct research areas. Cutting-edge antibody production services allow researchers the flexibility to create a custom antibody to meet their specific research needs.

  • Agdia - Easy-to-use test kits, reagents and laboratory testing services for agricultural diagnostics

  • AgriSera - Antibodies to mammalian, insect and bacterial antigens as well as antibodies in the area of photosynthetic research.

  • Ah Receptor (AhR) - Detection of Ah Receptor (AhR) in Formalin-Fixed, Paraffin-Embedded Rat Tissue

  • Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates - We report here the superior, largely unparalleled fluorescence emission and photostability of conjugates of a new series of sulfonated compounds named Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes. The number in each of their names refers to the approximate excitation maxima of the dyes, which nearly match the principal wavelength of several commonly used excitation sources. All of the Alexa dyes exhibit pH-insensitivity over a very broad range, and those that absorb at wavelengths greater than 480 nm have the high molar extinction coefficient typical of fluoresceins or rhodamines.

  • Alexis - Apoptosis, Cell Cycle & DNA Transactions, Combinatorial Chemistry Reagents, Cytoskeletal Research, Growth Factors, Cytokines & Chemokines, Immunology & Cancer Research, Neurochemicals, Nitric Oxide & Oxidative Stress, Signal Transduction.

  • Alomone Labs - Supplying innovative biochemicals which serve the neuroscience research community.

  • Alpha Diagnostic International - Alpha Diagnostic Intl., Inc., (ADI) located in San Antonio, Texas, USA, is one of the major providers of custom services such as peptides synthesis, and polyclonal antibodies. ADI maintains an USDA inspected and approved, animal facility. We have NIH/OPRR Assurance on file. Other antibody related services (affinity purification, conjugation to enzymes, dyes, fluorochromes, etc, ELISA, Western blots) are also provided.

  • ALT Inc - Novel, site specific nucleotide based photoaffinity reagents for labeling of antibodies

  • American Qualex - Labeled primary and secondary antibodies in addition to antibodies for signal transduction.

  • American Research Products - Mono- and polyclonal Antibodies, Antigens and Tissue Arrays

  • Amplification Methods to Increase the Sensitivity of In Situ Hybridization - Here we outline the principles of tyramide signal amplification using the catalyzed reporter deposition (CARD) technique, present practical suggestions to efficiently enhance the sensitivity of ISH with CARD, and discuss some applications and possible future directions of in situ nucleic acid detection using such an amplification strategy.

  • Amylase - Identification of Amylase (C-20) in Formalin-Fixed, Paraffin-Embedded Rat Tissue

  • An Optimized Method for ISH with Signal Amplification That Allows the Detection of Rare mRNAs - Using biotinyl–tyramide and streptavidin conjugated to alkaline phosphatase (AP) to introduce an additional round of amplification, we have now achieved a considerable enhancement of signal intensity relative to standard chromogenic ISH detection systems. We term this protocol TSA-AP (tyramide signal amplification using AP). In addition, we have optimized critical parameters to improve hybridization with digoxigenin (DIG)-labeled probes and to reduce nonspecific binding of probes, both strategies being essential for successful detection of rare mRNAs.

  • AnaSpec - Life science company providing innovative products (peptides, antibodies, fluorescent reagents, assay kits, synthesis resins and reagents) and custom manufacturing. AnaSpec offers a broad product line of these biochemicals and reagents for basic research, high-throughput screening and drug discovery. In conjunction, AnaSpec provides premier custom services including peptide synthesis, antibody production, and assay development.

  • Anaspec - An international manufacturer of antibodies in the areas of apoptosis, cancer research, and signal transduction; and secondary antibodies.

  • Anawa - Custom antibody production and a large selection of antibodies

  • Ancell - Anti-CD antibodies and more

  • Anderson Lab In Situ Hybridization Protocols - In Situ Hybridization of Frozen Sections, Whole Mount In Situ Hybridization, In Situ Hybridization on Cultured Cells and Additional Techniques

  • AngioBio - Provider of reagents for angiogenesis and lymphangiogenesis research, including many antibodies.

  • Anogen - Antibodies to cytokines, viral proteins, tumor markers and growth factors; and custom monoclonal antibody services.

  • Anti-Human CD Antibodies - Anti-Human CD Clustered Antibodies

  • Anti-Mouse CD Antibodies - Anti-Mouse CD Clustered and other Antibodies

  • Anti-Rat CD Antibodies - Anti-Rat CD Clustered and other antibodies

  • Antibodies - Structure and Sequence - This page attempts to summarise useful information on antibody structure and sequence. It provides a query interface to the Kabat antibody sequence data, general information on antibodies and crystal structures and links to other antibody-related information.

  • Antibodies Incorporated - Antibodies Incorporated is owned and operated by employees who have many years of experience in animal care, antiserum production, immunochemistry and related services applicable to aiding the academic or industry research scientist to save time and money in pursuit of their scientific goals. Having developed and manufactured our own diagnostic kits for many years has given us experience that is highly regarded in developing new assays and for contract manufacturing.

  • Antibody Directory - We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive.

  • Antibody Purification Handbook - The purpose of this handbook is to present the most effective and most frequently used strategies for sample preparation and purification of the many different forms of antibodies and antibody fragments used in the laboratory.

  • Antibody Staining of Drosophila Adult Retina - The following protocol has been adapted from Larry Zipursky's lab. It detects antigen in retinas that have been sectioned in plastic.

  • Antibody Structure - Antibody Structure - nicely illustrated

  • AntibodyShop - Large selection of monoclonal antibodies available online. The main antigens are: mannan binding lectin, complement system components, blood coagulation cascade components, immunoglobulins, albumins, cytokines, hormones, enzymes, haptens, human plasma proteins, and bacterial antigens.

  • Antigen Retrieval by Heating En Bloc for Pre-fixed Frozen Material - This article describes a simple alternative method for AR that can be used for aldehyde-fixed frozen sections.

  • Antigen Retrieval Immunohistochemistry - Antigen Retrieval Immunohistochemistry: Past, Present, and Future

  • Antigen Retrieval in Prion Protein Immunohistochemistry - Many different protocols for the detection of prions in brain tissue have been used. Thus far, picric and/or formic acid, steam autoclaving at 121C of sections, microwave treatment, and 4 M guanidine thiocyanate treatment have been suggested. The objective of our experiment was to obtain the standard pretreatment(s) resulting in optimal immunostaining. In the experiment, successive tissue slides of brain specimens of several Creutzfeldt–Jakob disease and control patients were stained using different combinations of pretreatments.

  • Antigen Retrieval of Basement Membrane Proteins from Archival Eye Tissues - Antigen retrieval (AR) methods can unmask tissue antigens that have been altered by fixation, processing, storage, or resin interactions. This is particularly important in the study of archival tissues, because primary fixatives and storage times may vary among specimens.

  • Antigen Retrieval Techniques - Antigen Retrieval Techniques: Current Perspectives

  • Antigen Retrieval Trial for Post-embedding Immunoelectron Microscopy by Heating - Antigen Retrieval Trial for Post-embedding Immunoelectron Microscopy by Heating with Several Unmasking Solutions

  • APC Formalin-Fixed Paraffin-Embedded MouseTissue - Identification of Adenomatous Polyposis Coli in Formalin-Fixed Paraffin-Embedded MouseTissue

  • Apotech - Antibodies for apoptosis and inflammation research

  • Application of Microwave Technology - Application of Microwave Technology to the Processing and Immunolabeling of Plastic-embedded and Cryosections

  • Applied Immunohistochemistry & Molecular Morphology - Applied Immunohistochemistry & Molecular Morphology provides complete coverage of the diagnostic and prognostic applications of immunohistochemistry (IHC), its contributions to the understanding of the biology of tumors and other lesions, and the technical aspects of setting up a laboratory and interpreting results.

  • Aptec Diagnostics - Polyclonal antibodies for use in quantitative turbidimetric and nephelometric determinations in human serum

  • Aquatic Diagnostics - Monoclonal antibodies for aquaculture. Antibodies to detect pathogens in fish and IgM in different fish species.

  • Araclon Biotech - Makers of 3 polyclonal antibodies: AB1 recognizes both Ab40 and Ab42, AB2 recognizes specifically Ab40, and AB3 recognizes specifically Ab42. They also make an ELISA kit to detect beta-amyloid protein in blood.

  • Argene Biosoft - Argene: development and production of diagnostic and/or research use reagents in the microbiology field by using immunology or molecular biology methods

  • Arista Biologicals - Monoclonal and polyclonal antibodies, and colloidal gold conjugates for fertility, infectious diseases, drugs of abuse, etc., as well as antigens for rapid diagnostic tests.

  • ASMA - 1A4 - ASMA (1A4) Optimax Immunostainer

  • ASMA - 1A4 - ASMA (1A4) Protocol for Techmate. NordiQC

  • ASMA - 1A4 - ASMA(1A4) Protocol for Autostainer. NordiQC

  • Assay Designs - Antibodies for research and diagnostic purposes (Immunoassays and Kits)

  • Assessment of Methods for Tissue-Based Detection of the HER-2/neu Alteration in Human Breast Cancer - Assessment of Methods for Tissue-Based Detection of the HER-2/neu Alteration in Human Breast Cancer: A Direct Comparison of Fluorescence In Situ Hybridization and Immunohistochemistry

  • Athens Research & Technology - Polyclonal sera to purified human proteins.

  • Aurion - AURION is a research oriented company dedicated to and specializing in the development and production of Ultra Small ImmunoGold Reagents, Silver Enhancement Reagents, Blocking Reagents, Incubation Solutions and Conventional ImmunoGold Reagents

  • Autoradiography for mRNA detection in mouse embryo tissue sections - Protocols from Oxford Practical Approach Series

  • Aves Labs - Specializing in chicken antibodies. They sell blocking reagents (BlokHen®) and reagents designed for immunoprecipitation (PrecipHen®) of chicken antibodies; a variety of enzyme- and fluorescence-tagged anti-chicken antibody reagents; and chicken antibodies against epitope tags such as c-myc, hemagglutinin, and FLAG.

  • Aviva Systems Biology - Aviva Systems Biology (ASB) develops, manufactures and markets reagent tools for gene transcription regulation research. We are best known for high quality novel antibodies, Innovative innovative Gene Regulatory Elements mapping and Analysis (GREMA) Technology and reliable custom gene transcription regulation mapping services

  • B-Catenin - Identification of B-Catenin in Formalin-Fixed, Paraffin Embedded Rodent Tissue

  • Bachem - Offers a comprehensive selection of sevices to meet your needs in immunochemicals of your choice.

  • Bcl-2 - Bcl-2 clone 124 staining protocol for Ventana Nexes. NordiQC

  • Bcl-2 - Bcl-2 clone bcl-2/100/D5 Staining protocol for Autostainer, DakoCytomation. NordiQC

  • Bcl-2 - Bcl-2 clone 100 Staining Protocol for I6000, BioGenex. NordiQC

  • Bcl-2 - Bcl-2 clone 8C8 Staining Protocol for LabVision Immunostainer. NordiQC

  • BCL2 - BCL2 Protocol for Autostainer

  • BCL2 - BCL2 Protocol for Techmate. NordiQC

  • BCL6 - BCL6 Protocol for Autostainer. NordiQC

  • BD Bioscience Clontech - Provider of GFP, GAL4, cell cycle, phosphoamino acid, phosphokeratin, and other antibodies, as well as many other innovative molecular and cell biology-related products

  • BD Biosciences - Immunocytometry systems

  • Beckman Coulter - Monoclonal and polyclonal antibodies to human and animal antigens.

  • Beecher Instruments - Beecher develops microarray technologies that facilitate high-throughput genomic and proteomic research. Offers automated and manual tissue microarray instruments and accessory tissue arrayer products.

  • Beta Polymerase - Identification of Beta Polymerase by an 8K Domain Antibody in Paraffin Embedded Rodent Tissue

  • Bethyl Laboratories - Primary and secondary antibodies to human, rodent and domestic animal serum proteins, affinity purified and conjugated, epitope tags, ELISA kits

  • Biocare Medical - BIOCARE is a unique manufacturer and supplier of immunostaining systems, histopathology products, monoclonal and polyclonal antibodies and automated staining equipment.

  • BioCore - BioCore supplies over 10,000 research related products including Antibodies, Immunoassay Kits, Enzymes, Immunotoxins, Reagents, and much more.

  • BioCytex - Thrombosis and Haemostasis field

  • Biodesign International - Broad range of antigens and antibodies

  • Biogenesis - Operating from the South Coast of England and New Hampshire in the US we are able to offer a wide range of Custom Services, in addition to our comprehensive catalogue of over 6,000 products.

  • BioGenex - BioGenex offers a broad range of reagents, detection kits, antibodies, probes, special stains, tissue microarrays, automated staining and imaging systems to streamline and standardize cellular and molecular analysis.

  • Biognostik - Supplier of custom-designed, high-quality ANTISENSE sequences and HybriProbes™ for in situ hybridization.

  • BioLegend - We offer products covering the areas of cell immunophenotyping, cytokines and chemokines, adhesion, phosphorylation, cancer research, cell-cycle analysis, and apoptosis. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications including flow cytometry, ELISA, immunoprecipitation, Western blotting, immunofluorescence microscopy, immunohistochemistry, and in vitro / in vivo functional assays.

  • BioLink International - Provide Recombinant HIV, HCV and Syphilis antigens for purpose of research and manufacturing. We also provide laboratory consumables to distributors, hospitals and research communities.

  • Biomarket - Biomarket offers more than 100 products in the field of infectious diseases, monoclonal antibodies, antigens, extracellular matrix, integrin and molecular biology products including modifying enzymes and PCR reagents.

  • BioMed Central - BioMed Central. Over 100 Open Access journals covering all areas of Biology and Medicine

  • Biomeda - Full range of detection kits, a diversified line of monoclonal and polyclonal antibodies,

  • Biomedica - Supplier of endothelin and big endothelin antibodies.

  • Biomedical Technologies - BTI prepares over 35 different antibodies. Antibodies to the cytoskeletal proteins, growth & attachment factors, lipoproteins, hormones and keratinocyte differentiation markers are also offered. All our products are produced in the USA. Labeled second antibodies and precipitating antibodies are available in convenient packaging.

  • BIOMOL International - BIOMOL offers a range of biochemicals with applications in signal transduction, immunohistochemistry, gene regulation, apoptosis, neuroscience and drug discovery. In addition to purified enzymes, antibodies, and assay kits, BIOMOL’s innovative products include Nupherin-neuron transfection reagent, Fluor de Lys HDAC assay kit, and Screen-Well compound libraries. www.biomol.com 800-942-0430

  • Biomol International - The BIOMOL International worldwide network offers specialised reagents for signal transduction research (including antibodies, peptides, enzymes, enzyme activators and inhibitors, second messengers, and assay kits as well as a comprehensive offering of bioactive lipids and neuropharmacologics) and a range of compound libraries for screening (previously available from BIOMOL Research Laboratories) with the innovative products and services for cell biological research available from AFFINITI. This includes a wide range of products for neuroscience research and the world's most comprehensive range of substrates, inhibitors, enzymes, proteins and antibodies for use in ubiquitin, proteasome and COP9-signalosome research. AFFINITI staff also specialise in the manufacture of high quality synthetic peptides and in the synthetic peptide approach to protein-reactive antibody production.

  • BioProcessing - Antibodies to Ca15-3, Ca27.29, breast tumor antigens, Ca125, ovarian tumor antigen, Ca19-9, GI tumor antigen, CEA, carcinoembryonic antigen, AFP, alpha fetoprotein, Ca72.4, Tag-72, NSE, neuron specific enolase, Cyfra21-1, cytokeratin-19, PSA, prostate specific antigen using cell culture technologies.

  • Biosense Laboratories - Producers of antibodies and ELISA kits for the detection of biomarker proteins in fish. Products include monoclonal and polyclonal antibodies for the detection of responses to oil hydrocarbons, PAHs, PCBs, dioxins, endocrine disruptors, etc...

  • Biosonda - Featuring anti-human acrosin and anti-gizzerosine antibodies and Blue Carrier, a highly soluble and purified carrier protein from the hemocyanin family

  • Biosource - Biosource International, Inc. is a broad based life sciences company focused on providing solutions in the areas of functional proteomics and advanced drug discovery through the development, manufacturing, marketing, and distribution of unique biologically active reagent systems which facilitate, enable, and accelerate pharmaceutical development as well as biomedical research.

  • BioSource International - Develops, manufactures, markets and distributes monoclonal and polyclonal antibodies, recombinant proteins, custom oligonucleotides and quantitative cDNA amplification kits for the study of immunology and molecular biology

  • BiosPacific - Antibodies and antigens for the development of diagnostic assays

  • Biostride - East Coast Biologics - Monoclonal and polyclonal antibodies to drugs of abuse, environmental compounds, hormones and steroids, tumor markers, infectious diseases, therapeutic drugs, and other miscellaneous compounds

  • Biotin and Digoxigenin as Labels for Light and Electron Microscopy in Situ Hybridization Probes - Biotin and Digoxigenin as Labels for Light and Electron Microscopy in Situ Hybridization Probes: Where Do We Stand?

  • Biotinylation of Antibodies - Biotin is used in two-step detection systems in concert with conjugated avidin. Biotin is typically conjugated to proteins via primary amines (i.e., lysines). Usually, between 3 and 6 biotin molecules are conjugated to each antibody.

  • Biotrend - BIOTREND is specialised in Immunology, Neuroscience, Pharmacology and Radiochemistry, supplying biomedical and pharmaceutical research with a large range of antibodies, radiolabelled products, custom synthesis and antibody production services.

  • BioVendor Laboratory Medicine - Specializes in the production and distribution of in vitro research products for molecular and cellular laboratory medicine

  • BMA Biomedicals - Monoclonal antibodies, polyclonal antibodies, and ELISA kits for macrophage and inflammation typing in mouse, human and rats

  • Boston Biochem - Antibodies and assay kits for the ubiquitin proteasome pathway

  • BRDU Mouse - Detection of BRDU in formalin-fixed, paraffin-embedded mouse tissue

  • BRDU Rat - Detection of BRDU in formalin-fixed, paraffin-embedded rat tissue

  • Calbindin-D-28K in Formalin-Fixed Paraffin-embedded Mouse Tissue - Detection of Calbindin-D-28K in Formalin-Fixed Paraffin-embedded Mouse Tissue

  • Calbiochem - Distributor of an extensive range of monoclonal and polyclonal antibodies, secondary antibodies and their conjugates, plus many accessory products for immunoassay and immunoseparation.

  • Calcium-induced Modification of Protein Conformation Demonstrated by Immunohistochemistry - Calcium-induced Modification of Protein Conformation Demonstrated by Immunohistochemistry: What Is the Signal?

  • Calgranulin in Formalin-Fixed, Paraffin Embedded Rat Tissue - Identification of Calgranulin in Formalin-Fixed, Paraffin Embedded Rat Tissue

  • Calretinin - Calretinin Protocol for DakoCytomation Horizon. NordiQC

  • Calretinin clone 5A5 - Calretinin clone 5A5 Protocol for DakoCytomation Autostainer. NordiQC

  • Calretinin in Formalin-Fixed, Paraffin Embedded Rodent Tissue - Identification of Calretinin in Formalin-Fixed, Paraffin Embedded Rodent Tissue

  • Caltag Laboratories - Antibodies to cell markers for flow cytometric applications

  • Cambio - STAR*FISH Chromosome Painting

  • CanAg Diagnostics - A biotechnology company specialising in the development of specific reagents (monoclonal antibodies) and laboratory tests that can be used for sensitive and specific determination of tumor markers and biochemical markers of brain disease

  • Capralogics - Capralogics produces high quality polyclonal antibodies. Chemokine Receptors, Interleukins, Nuclear Components, Apoptosis, Cell Membrane, Protein Kinases, Signaling Intermediates, Lymphocyte Signaling

  • Capricorn - Manufacturer and distributor of immunodiagnostic products including monoclonal and polyclonal antibodies to infectious diseases, hormones. steroids, tumor markers, and serum proteins.

  • Carcinoembryonic antigen - CEA - Carcinoembryonic antigen (CEA) Protocol for DakoCytomation Autostainer

  • Caspase-3 - Detection of Cleaved Caspase-3 in Formalin-Fixed, Paraffin-Embedded Human and Rodent Tissue

  • Catalase in Formalin-Fixed, Paraffin Embedded Rat Tissue - Detection of Catalase in Formalin-Fixed, Paraffin Embedded Rat Tissue

  • Cayman Chemical - Cayman Chemical Company is helping make research possible by supplying scientists worldwide with biochemical tools in research disciplines such as cancer, nitric oxide, neuroscience, apoptosis, oxidative injury, endocrinology, and much more. We specialize in assay kits for the measurement of eicosanoids, free radical biomarkers, cyclic nucleotides, cytokines, hormones and nitric oxide. In addition, Cayman offers a broad range of quality biochemicals including eicosanoids, nitric oxide reagents, and a variety of related lipids, fatty acids, enzymes, and antibodies.

  • CC10 using LSAB Kit in Formalin-Fixed, Paraffin Embedded Rodent Tissue - Identification of CC10 using LSAB Kit in Formalin-Fixed, Paraffin Embedded Rodent Tissue

  • CCK-A Receptor - Detection of CCK-A Receptor (G-17) in Formalin-Fixed, Paraffin-Embedded Rat Tissue

  • CD10 - CD10 clone 56C6 Protocol for DakoCytomation Autostainer. NordiQC

  • CD10 - CD10 clone 56C6 Protocol for DakoCytomation Autostainer. NordiQC

  • CD10 is expressed in a subset of chromophobe renal cell carcinomas - CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas.

  • CD117 - CD117 (polyclonal) Staining Protocol for Autostainer

  • CD117 - CD117 (polyclonal) Staining Protocol for Ventana BenchMark XT

  • CD117 c-kit - CD117 (c-kit) Polyclonal, Protocol for DakoCytomation Autostainer. NordiQC

  • CD117 c-kit - CD117 (c-kit) Polyclonal, Protocol for DakoCytomation TechMate

  • CD117 c-kit - CD117 (c-kit) Polyclonal, Protocol for Ventana BenchMark. NordiQC

  • CD117 c-kit - CD117 (c-kit) Polyclonal, Protocol for DakoCytomation TechMate. NordiQC

  • CD14 - CD14 clone 7 Protocol

  • CD14 - CD14 clone 7 Protocol for TechMate

  • CD15 - CD15 clone MMA Protocol for DakoCytomation Autostainer. NordiQC

  • CD15 - CD15 clone Tu9 Protocol for GenoMx i6000, BioGenex. NordiQC

  • CD15 - CD15 clone MMA Staining Protocol for TechMate 500

  • CD15 - CD15 clone MMA Staining Protocol for Ventana ES

  • CD15 - CD15 clone C3D-1 Staining Protocol for TechMate 500

  • CD15 C3D-1 - CD15(C3D-1) Protocol for Autostainer LabVision

  • CD19 - Detection of CD19 in Frozen Mouse Tissue

  • CD20 - CD20 clone L26 Protocol for DakoCytomation Horizon. NordiQC

  • CD20 Expression in Hodgkin and Reed-Sternberg Cells of Classical Hodgkin’s Disease - CD20 can be expressed in Hodgkin and Reed-Sternberg (HRS) cells of classical Hodgkin’s disease (HD), but its clinical significance remains controversial. Therefore, we correlated CD20 expression with presenting features and clinical outcome of untreated patients with classical HD.

  • CD23 - CD23 clone 1B12 Protocol for DakoCytomation Autostainer. NordiQC

  • CD23 - CD23 clone 1B12 Protocol for DakoCytomation Autostainer. NordiQC

  • CD23 - CD23 clone 1B12 Protocol for Ventana NexES. NordiQC

  • CD23 - CD23 clone MHM6 Protocol for DakoCytomation Autostainer. NordiQC

  • CD3 - CD3 clone PS1 Protocol for DakoCytomation Autostainer. NordiQC

  • CD3 - CD3 clone F7.2.38 Staining Protocol for DakoCytomation Autostainer.

  • CD3 - CD3 clone PS1 Staining Protocol for Ventana Benchmark.

  • CD3 - CD3 clone PS1 Staining Protocol for DakoCytomation Autostainer

  • CD3 - CD3 clone SP7 Manual Staining Protocol

  • CD3 - CD3 (polyclonal) Staining Protocol for LabVision Autostainer

  • CD3 - CD3 (polyclonal) Staining Protocol for LabVision Immunostainer

  • CD3 - Detection of CD3 in Formalin-Fixed, Paraffin-Embedded Human and Mouse Tissue

  • CD3 Polyclonal - CD3 Polyclonal Protocol for Dako TechMate

  • CD30 - CD30 clone Ber-H2 Protocol for DakoCytomation Autostainer. NordiQC

  • CD30 - CD30 clone Ber-H2 Protocol for DakoCytomation TechMate. NordiQC

  • CD30 - CD30 clone Ber-H2 Protocol for Ventana NexES. NordiQC

  • CD30 - CD30 clone Ber-H2 Protocol for DakoCytomation TechMate

  • CD30 - CD30 clone Ber-H2 Protocol for Ventana BenchMark. NordiQC

  • CD31 - CD31 clone JC/70A Protocol for DakoCytomation Autostainer. NordiQC

  • CD31 - CD31 clone JC/70A Protocol for DakoCytomation TechMate. NordiQC

  • CD31 - CD31 clone JC/70A for Autostainer. NordiQC

  • CD31 - CD31 clone JC/70A Protocol for Ventana BenchMark. NordiQC

  • CD34 - CD34 clone QBEnd 10 Protocol for Labvision Autostainer. NordiQC

  • CD34 - CD34 clone My10 Protocol for DakoCytomation TechMate

  • CD34 - CD34 clone QBEnd 10 manual protocol. NordiQC

  • CD34 - CD34 clone QBEnd 10 for DakoCytomation Autostainer. NordiQC

  • CD4 - CD4 clone 1F6 Staining Protocol for Ventana NexES

  • CD4 - CD4 clone 1F6 Staining Protocol for TechMate

  • CD4 - CD4 clone 4B12 Staining Protocol for DakoCytomation Autostainer

  • CD4 - CD4 clone 4B12 Staining Protocol for DakoCytomation TM500

  • CD40 in Formalin Fixed Paraffin Embedded Mouse Tissue - Detection of CD-40 in Formalin Fixed Paraffin Embedded Mouse Tissue

  • CD40 in Frozen Mouse Tissue - Detection of CD-40 in Frozen Mouse Tissue

  • CD5 4C7 - CD5 (4C7) Protocol for Autostainer Lab Vision. NordiQC

  • CD5 4C7 - CD5 (4C7) Protocol for Ventana NexES. NordiQC

  • CD5 CD5/54/F6 - CD5 (CD5/54/F6) Protocol for DakoCytomation Autostainer

  • CD68 - CD68 clone KP1 for DakoCytomation Autostainer. NordiQC

  • CD68 - CD68 clone KP1 Protocol for Ventana NexES. NordiQC

  • CD68 - CD68 clone PG-M1 for Ventana NexES. NordiQC

  • CD68 - CD68 clone PG-M1 Protocol for DakoCytomation Autostainer. NordiQC

  • CD79a - CD79a clone JCB117 Protocol for LabVision Autostainer. NordiQC

  • CD79a - CD79a clone JCB117 for Ventana NexES. NordiQC

  • CD8 - CD8 clone 1A5 Staining Protocol for Ventana BenchMark

  • CD8 - CD8 clone 4B11 Staining Protocol for TechMate 500

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