Immunohistochemistry - In Situ Hybridization

Appendix, tonsil, pancreas and liver (Page 1)

A skin punch biopsy needle can be used to cut out representative tissue from preferable normal tissue blocks.
Composition, size and number may vary, but 2-6mm cores are normally sufficient.
Optimally, a 6mm core is replaced by several 2-4mm cores to cover weak, intermediate and strong expression of antigen.

Following Multi Tissue Control Blocks are suggestions from Nordic Immunohistochemical Quality Control NordiQC

Multi Tissue Blocks:

Appendix, tonsil, pancreas and liver page 1

Appendix, tonsil, pancreas and liver page 2

Brain, striated muscle, skin and melanoma

Lung, thyroid, prostate and placenta

Thymus, bone marrow, Hodgkin lymphoma and tonsil

	APPENDIX	TONSIL	PANCREAS	HEPAR
AAT Alfa-1-antitrypsin (Cytopl)	Macrophages & villi of columnar epithelial cells	Macrophages	0	Kupfer cells & hepatocytes (globuli)
ASMA Alfa-smooth muscle actin (Cytopl)	Smooth muscle cells in vessels and in muscle layers. Myofibroblasts lining the epithelial surface	Smooth muscle cells in vessels	Smooth muscle cells in vessels	Smooth muscle cells in sinusoids
B-CATENIN Beta-catenin (Membrane)	Membranes of columnar epithelial cells. Endothelial and follicular denritic cells	Membranes of squamous epithelial cells	Membranes of acinar epithelial cells (ducts) and endocrine cells	Hepatocytes - weak membranous
BCL2 Bcl2-oncoprotein (Cytopl)	All peripheral lymphocytes incl T-cells in germinal centres -Germinal centre B-cells are negative.	All peripheral lymphocytes incl T-cells in germinal centres -Germinal centre B-cells are negative.	Weak reaction of epithelial cells.	0
BCL6 Bcl6-protein (Nuclear)	Germinal centre B-cells	Germinal centre B-cells and basal squamous cells	0	0
BOB.1 BOB.1-protein (Nuclear)	Strong reaction in germinal centre B-cells -weak reaction in mantle zone B-cells and plasma cells	Strong reaction in germinal centre B-cells-weak reaction in mantle zone B-cells and plasma cells	0	0
CD2 (Membrane)	All T-cells -Isolated T-cell in germinal centres	All T-cells -Isolated T-cells in germinal centres	T-cells	T-cells
CD3 (Membrane)	All T-cells -Isolated T-cell in germinal centres	All T-cells -Isolated T-cell in germinal centres	T-cells	T-cells
CD4 (Membrane)	60 - 80 % of T-cells	60 - 80 % of T-cells and Germinal centre macrophages	0	Kupfercells and sinusoid endothelial cells
CD5 (Membrane)	All T-cells -Isolated T-cell in germinal centres	All T-cells -Isolated T-cells in germinal centres	T-cells	T-cells
CD7 (Membrane)	Almost all T-cells	Almost all T-cells	T-cells	T-cells
CD8 (Membrane & cytopl)	20 - 40 % of the T-cells and NK-cells, Isolated T-cells in germinal centres	20 - 40 % of the T-cells and NK-cells, Isolated T-cells in germinal centres	0	0
CD10 (Membrane)	Germinal centre B-cells, neutrophile granulocytes	Germinal centre B-cells, neutrophile granulocytes	0	Gall canaliculi
CD14 (Membrane)	Follicular dendritic cells and macrophages	Follicular dendritic cells and macrophages	Macrophages	Kupfercells and sinusoid endothelial cells
CD20 (Membrane)	All B-cells incl germinal centre B-cells, plasma cells are neg.	All B-cells incl germinal centre B-cells, plasma cells are neg.	B-cells	B-cells
CD21 (Membrane)	Follicular dendritic cells	Follicular dendritic cells and a few mantle zone B-cells	0	0
CD23 (Membrane)	Follicular dendritic cells and 10 - 50 % of the mantle zone B-cells	Follicular dendritic cells and 10 - 50 % of the mantle zone B-cells	0	0
CD31 (Membrane & cytopl)	Endothelial cells and a few mantle zone B-cells	Endothelial cells and a few mantle zone B-cells	Endothelial cells	Endothelial cells
CD34 (Membrane & cytopl)	Endothelial cells and myofibroblasts	Endothelial cells and myofibroblasts	Endothelial cells and myofibroblasts	Portal endothelial cells
CD38 (Membrane)	Germinal centre B-cells and plasma cells	Germinal centre B-cells and plasma cells	Plasma cells	Plasma cells and weak reaction of sinusoids
CD43 (Membrane)	Almost all T-cells	Almost all T-cells -Isolated T-cells in germinal centres	T-cells	T-cells
CD45 Leucocyt common antigen (Membrane)	All lymphocytes	All lymphocytes	All lymphocytes	Lymphocytes and Kupfer cells
CD56 (Membrane)	Peripheral nerves -ganglion cells and axons. Endocrine cells	NK-cells in the interfollicular areas	Peripheral nerves and the islets of Langerhans	Peripheral nerves
CD57 (Membrane)	Peripheral nerves -ganglion cells and axons. Endocrine cells	NK-cells in the interfollicular areas and T-cells in germinal centres	Peripheral nerves and a few cells in the islets of Langerhans	0
CD68 (Membrane & cytopl)	Granulocytes and germinal centre macrophages	Granulocytes and germinal centre macrophages	Granulocytes	Kupfer cells
CD79 (Membrane & cytopl)	All B-cells incl germinal centre cells and plasma cells	All B-cells incl germinal centre cells and plasma cells	All B-cells	All B-cells
CD117 (Membrane & cytopl)	Mast cells and Cajal cells- smooth muscle cells shall be negative	Mast cells	Mast cells	Mast cells
CD138 (Membrane & cytopl)	Plasma cellsand a weak reaction of the columnar epithelial cells	Plasma cells	Plasma cells	Plasma cells
Cdx2 (Nuclear)	All nuclei of columnar epithelial cells	Negative	Weak reaction of epithelial cells in large ducts	Negative - occasionally few gall epithelial cells are pos.
CEA Carcino-embryonic antigen, mono (Cytopl)	All columnar epithelial cells - luminal accentuation and reaction of the cytoplasm	Squamous epithelial cells	0	0
CEA PO Carcino-embryonic antigen, poly (Cytopl)	All columnar epithelial cells - luminal accentuation and reaction of the cytoplasm	Squamous epithelial cells, granulocytes and macrophages	Granulocytes and macrophages	Gall canaliculi and kupfer cells
CGA-PO Chromogranin A, poly (Cytopl)	Endocrine cells and peripheral nerves -ganglion cells and axons	0	Endocrine cells in the islets of Langerhans	Peripheral nerves.
CGA Chromogranin A, mono (Cytopl)	Endocrine cells and peripheral nerves -ganglion cells and axons	0	Endocrine cells in the islets of Langerhans	Peripheral nerves.
CK5 cytokeratin 5 (Cytopl)	Mesothelial cells	Squamous epithelial cells.	Negative	Negative
CK5/6 Cytokeratin 5/6 (Cytopl)	Mesothelial cells	Squamous epithelial cells.	Negative	Negative
CK7 Cytokeratin 7 (Cytopl)	Mesothelial cells -occasionally a few columnar epithelial and endothelial cells.	Squamous epithelial cells	Columnar epithelial cells of large ducts and intercalated ducts	Gall columnar epithelial cells
CK8 Cytokeratin 8 (Cytopl)	Columnar epithelial cells & mesothelial cells	A few squamous epithelial cells and focally fibroblastic reticulum cells	All columnar epithelial cells and islets of Langerhans	Gall columnar epithelial cells & hepatocytes
CK13 Cytokeratin 13 (Cytopl)	Negative	Suprabasal squamous epithelial cells.	Negative	Negative
CK14 Cytokeratin 14 (Cytopl)	Mesothelial cells	Squamous epithelial cells.	Negative	Negative
CK18 Cytokeratin 18 (Cytopl)	Columnar epithelial cells & mesothelial cells	Few squamous epithelial cells and focally fibroblastic reticulum cells	All columnar epithelial cells and islets of Langerhans	Gall columnar epithelial cells & hepatocytes
CK19 Cytokeratin 19 (Cytopl)	Columnar epithelial cells & mesothelial cells	Few squamous epithelial cells	Columnar epithelial cells of large ducts	Gall columnar epithelial cells
CK20 Cytokeratin 20 (Cytopl)	Columnar epithelial cells and endocrine cells	0	0	0
CK Sentinel node mamma Cytokeratin 7 + 19 (Cytopl)	Columnar epithelial cells & mesothelial cells. Occasionally endothelial cells.	Few squamous epithelial cells	Columnar epithelial cells of large ducts and intercalated ducts	Gall columnar epithelial cells
CK LMW (Cam5.2) Low mol weight cytokeratin (Cytopl)	Columnar epithelial cells & mesothelial cells	A few squamous epithelial cells and focally fibroblastic reticulum cells	All columnar epithelial cells and islets of Langerhans	Gall columnar epithelial cells & hepatocytes
CK HMW (34BE12) High mol weight cytokeratin (Cytopl)	Negative -A few columnar epithelial cells might be pos.Mesothelial cells are pos.	Squamous epithelial cells	Negative - A few columnar epithelial cells (ducts) might be pos	Negative -gall epithelial cells can be pos.
CK Pan (AE1/AE3) Pan cytokeratin (Cytopl)	Columnar epithelial cells & mesothelial cells	Squamous epithelial cells and focally fibroblastic reticulum cells)	All columnar epithelial cells and islets of Langerhans	Gall columnar epithelial cells & hepatocytes
CK Pan (KL1) Pan cytokeratin (Cytopl)	Columnar epithelial cells & mesothelial cells	Squamous epithelial cells and focally fibroblastic reticulum cells)	All columnar epithelial cells and islets of Langerhans	Gall columnar epithelial cells & hepatocytes
CK Pan (MNF116) Pan cytokeratin (Cytopl)	Columnar epithelial cells & mesothelial cells	Squamous epithelial cells and focally fibroblastic reticulum cells)	All columnar epithelial cells and islets of Langerhans	Gall columnar epithelial cells & hepatocytes
COL IV Collagen IV (Extra cel)	Basal membrane under the epithelial surface and muscle cells	Basal membrane under the epithelial surface	Muscle cells	Muscle cells
CR Calretinin (Cytopl & nuclear)	Peripheral nerves -ganglion cells and axons - ,macrophages and mesothelial cells.	Macrophages	Peripheral nerves -ganglion cells and axons -, macrophages	0
CYD1 Cyclin D1 (Nuclear)	Few basal columnar epithelial cells	Parabasal squamous epithelial cells andfew fibroblastic cells	Few basal columnar epithelial cells	0

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