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I am a Clinical Researcher in a Medical Device company. IHC is not exactly my field of specialty and I would like to get some expert opinions on the following matter.
Our company is intending to use services from a commercial lab in order to determine effects of our product related to skin rejuvenation, anti ageing, healing activity and angiogenesis.
The following stainings, following treatments with the product, have been suggested:
Basl keratin (CK14)
Collagen III
Collagen IV
Glycosaminoglicans (GAG)
cells in mitosis (KI67)
endothelial cells (PECAM-1)
My questions are:
1. Are all those stainings relevant/necessary for the above mentioned purpose?
2. Are there better suited stainings available?
I am looking forward to getting your expert opinion.
Best regards,
Vlad P.
Sat Nov 10, 2007 9:00 am
ole
Rank: Member
Joined: Oct 11, 2004
Posts: 855
Post subject:
Perhaps you might consider this ab - Mitosis.
Works also well on human tissue. Easy working antibody in my hands and i guess Carl aswell.
Reg the rest of your antibodies - i might get back to it when i find a little more time or find any other suggestions.
Ole
Sat Nov 10, 2007 4:22 pm
ole
Rank: Member
Joined: Oct 11, 2004
Posts: 855
Post subject:
I am far from any expert, but........
CD31 (PECAM-1). Reg endothelial cells. CD31 also stain myeloid cells and NK cells and others, so there might be? much noise.
CD31 does not label if i remember correct lymfatic endothelial cells if that is important for your project.
CD34 (QBend10)labels eg lymfatic endothelial cells in addition, and most endothelial cells (not in the large vessels (most of them). Labels also eg fibrocytes which might give some noise in some tissues.
D2-40 (podoplanin) is a good lymfatic marker.
Also i find podocalyxin to be a decent endothelial marker.
Sat Nov 10, 2007 7:16 pm
ole
Rank: Member
Joined: Oct 11, 2004
Posts: 855
Post subject:
I have CK14 (neomarkers) LL002. In my hands a good ab and a decent marker for basal keratinocytes. It stains however suprabasalcells in some "conditions". I dont know if there are better antibodies, probably is?
There is IMO too little info in your post to give much info.
Hmm it was also mysterious difficult to switch from a diagnostic/disease approach to a "research" of the markers im familiar with.
Dont think I can help.
Confused Ole
Sun Nov 11, 2007 3:49 pm
Carl
Rank: Member
Joined: Oct 11, 2004
Posts: 1872
Post subject: Hi , Vlad
We thank you for being honest....
However, you ask for vital data. You will be paid for this...we will not.
The commercial lab that you have employed to do the work for you should have the best answers.
If you doubt them, ask them for Immunohistochemical pictures of the cells/tissues that they say they can demonstrate. Then, post them here....we can then comment on the quality of the pictures.
Sorry but, please do not use this excellent site for commercial reasons.
If you wish, talk to Hogne, the Administrator of this site. I will be guided by his answer.
Best wishes.
Carl
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