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Hi all,
Happy new year !
I was wondering about a basic Immunohistochemistry question. I would appreciate if someone could enlighten me regarding my question, which is as follows.
GAD (glutamic acid decarboxylase) is a precursor for GABA synthesis. Since GABA is a neurotransmitter it should be localized in the axon terminals and hence GAD staining should only stain GABA containing axon terminals. But in some papers, I found that GAD also stains interneuron dendrites. Why is this so? Does it mean that such dendrites also contain GABA? Are those types of dendrites that contain GABA ( if any) play role in neurotransmission?
Thanks in advance.
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Last edited by channels on Fri Jan 02, 2009 5:32 pm; edited 1 time in total
Thu Jan 01, 2009 1:26 pm
Carl
Rank: Member
Joined: Oct 11, 2004
Posts: 1871
Post subject: Happy New year!
Interesting Q, channels.
I have passed it on to our "GAD" people....
I placed two images in the gallery of GAD65/67 localisation in pwax sections ages ago......having just looked at them they are wrongly labelled as "rat" tissues. If Hogne would be kind enough to delete them, I would appreciate it.
I will now upload them correctly labelled as "mouse" sections.
Carl
Thu Jan 01, 2009 3:30 pm
channels
Rank: Member
Joined: Nov 12, 2007
Posts: 10
Post subject:
Thanks Carl for passing my question to GAD people. I hope I can get the answer from someone.
Cheers,
Channels
Fri Jan 02, 2009 5:33 pm
Carl
Rank: Member
Joined: Oct 11, 2004
Posts: 1871
Post subject: From Martin...
A very experienced PostDoc in our centre:
" Interneurones are predominantly inhibitory and GABAergic in nature - hence the staining for the enzyme GAD ( which isoform though?), I used to use GAD 67 as a more reliable marker of GABAergic neurones. These dendrites should contain GABA and ( at least in theory) be involved in neurotransmission - GABA however switches from being excitatory to inhibitory around birth, so depending on the type of neurones investigated these could either be inhibitory or exitatory.... http://www.jneurosci.org/cgi/reprint/6/2/541.pdf "
I tried to cutnpaste Martin's email but......I just kept getting a Paste of your original Q, channels.....weird, sigh!
So, any errors are mine!
Any problem accessing the pdf, just send me a PM here...
best wishes
carl
Tue Jan 06, 2009 8:28 pm
channels
Rank: Member
Joined: Nov 12, 2007
Posts: 10
Post subject:
Hi Carl,
Thanks a lot...Good one! This question was bothering me for quite some time.
Thanks to the "experienced postdoc" as well.
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