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Post subject: Protocol for IHC B-catenin rat bone?
Need help with a IHC protocol for B-catenin.
Have a protocol that works with chondrocytes and cartilage biospies, but now I can't get it to work with bone. Does anyone have any experience regarding IHC and bone? Does it have more autofluoresence then for example cartilage...
Wed Jan 11, 2012 6:52 pm
Carl
Rank: Member
Joined: Oct 11, 2004
Posts: 1872
Post subject: Hi DocT
Sorry that you have no response, yet.
Please give some details:
Tissue prep ?
Are you having problems with signal strength or....autofluorescence?
Can you post some images of your results so far?
I have posted a beta Cat image in developing muscle so I would be interested to see the location of the positivity you get in chondrocytes.
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