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Post subject: Immuohistochemistry of adipose tissue
Hi all,
I would like to do macrophage staining in sheep adipose tissue. I do not know where to start from? Any suggestions please !!!!!!!!!!
Prabhat
Wed Mar 14, 2012 9:29 pm
Carl
Rank: Member
Joined: Oct 11, 2004
Posts: 1871
Post subject: Hi again, Prabhat
I recall this:
process to Pwax. Cut sections. Float out. Mount onto Superfrost Plus slides ( or your own APES-treated/subbed slides). Stick in 60C oven for 1hr minimum ( I usually leave overnight)
Dewax, rehdrate,H2O2 block ( if doing Px IHC), perform whichever HIER works for you.
Wash in tapwater.
Block in buffer containing BSA.
Incubate in Iba1 ( from Wako)
Detect with any system you wish.
Will not fail.
A general stABCpx-DAB method is in Protocols
Carl
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