To Hrn: Red Chromogen?

Luisa · **Luisa**

Which red chromogen did you use for this triple labeling? Aqueous or permanent mounting medium?

Thanks a lot!

Luisa

http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=Triple+staining&g2_itemId=11597

Carl · **Carl**

He mentions Biocare so, alk phos chromogens here:
http://biocare.net/products/detection/chromogens/

It's a very nice image

ole · **ole**

I have used all of their substrates i think - especially their red chromogens.
In my hands - Warp red seems 1,5-2x more sensitive than VFR.

Uhm at least a brief dehydration (common dehydration) wont reduce the signal. I mount in permanent mounting medium (both for warp and vulcan), and altho different red chromogens reacts differently with different permanent mounting mediums - it doesnt seem to be a problem with Warp or Vulcan?
I just use warp, now. Its great Wink

ole

Immunohistochemistry	In Situ Hybridization	Immunostaining
Immunohistochemistry Images	Tissue staining	Pathology
Tumor Markers	IHC Reagents	Tissue Micro Array
Immunofluorescence Staining	Positive Control Slide	Polyclonal Antibody
Monoclonal Antibody	IHC Staining Protocol	In Situ Hybridization Images
Immunohistochemistry Protocol	Immunofluorescence	Immunohistochemical Staining
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