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Why do many use Triton or TWEEN in their Retrieval solution?
After all, we are retrieving antigen sites in FFPWS.
So, the alcohol and xylene will get rid of all/most hydrophobic lipids?
The remaining hydrophilic lipids will surely not need any Surfactant?
I am sincerely interested.
Anticipatingly,
Carlos
Sun Jun 17, 2012 7:03 pm
ole
Rank: Member
Joined: Oct 11, 2004
Posts: 853
Post subject: ??????????????
Idk
I thought perhaps adding tween etc might be benefitial for better rehydrating the tissue - better penetration of retrieval buffer - especially when using low molar retrieval soln? Some claimed that surfactant treatment eg 0,05 -0,25% saponin -30mins gave gave good results for some abs- and triton too for others - so perhaps it is to be on the safe side
I have compared (now and then) over the years - compared retrieval buffers with and without detergents - and found no significant difference - SOOoo far.
I am sincerely interested too - as many vendors use surfactants in their retrieval solutions.
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