Post subject: Why do we use serum on our sections?
most Abs that we buy are in buffer.
So...forget using Goat etc Serum to dilute your Abs.
Sure, if you make your own polyclonal Abs...then the preimmune serum is important...FROM THE SAME RABBIT, pre-immune;-)
Them days are gone...
Because many Abs are Immunopurified.
Also....no point in pre-adsorbing your Ab with immuno. peptide...it will always work.
Peptide is different from any Ag in a section of tissue.
You cannot post new topics in this forum You cannot reply to topics in this forum You cannot edit your posts in this forum You cannot delete your posts in this forum You cannot vote in polls in this forum