When you want to detect a specific DNA sequence in your tissues; I suspect that you have to go through a denaturation step to de-anneal the native DNA so that the probe can bind them instead.
From what I gathered, this is realized by heating up the tissues (similar to the denaturation step in a classic PCR)
So, I was wondering if I understood it right and if there was other methods.
Most of the references I could find seemed more interested in the detection of RNA.
It might seem like a silly question, but I am supposed to teach a class about in situ even if I have very little experience with this technique and I would like not to teach them too much wrong informations. This fills me up with nervousness and also with questions...
Tue Jan 29, 2008 6:47 pm
Joined: Oct 11, 2004
Post subject: How did the class go, Simon??
Yes, we apologise for not answering but, but....if YOU do not know, how can you teach?
Tue Feb 12, 2008 8:50 pm
Joined: Jan 16, 2008
Well... Lot of references.
Plus, it's not as much as I don't know, more than I picked most of it as I went and have a few knowledge gaps...
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