There are currently, 100 guest(s) and 0 member(s) that are online.
You are Anonymous user. You can register for free by clicking here
Login
Don't have an account yet? You can create one. As a registered user you have some advantages like theme manager, comments configuration and post comments with your name.
What I'm doing: I am using DIG oligo probes to label mRNA in fish retina.
I am trying to adapt available protocols to what suits my needs and I'm left with a few questions...
1. What is the purpose of the dehydration step and is it an essential one - or is it more important for chromosome labeling?
2. Is the addition of salmon sperm or tRNA a necessity (some protocols include it, others use protein blocks)?
3. I've noticed that probe lengths can vary widely. Are super short probes of ~20 bases ridiculously short?
You cannot post new topics in this forum You cannot reply to topics in this forum You cannot edit your posts in this forum You cannot delete your posts in this forum You cannot vote in polls in this forum
