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Immunohistochemistry - In Situ Hybridization: Forums

Immunoportal.com :: View topic - Newbie question
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moerae

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Joined: Apr 20, 2009

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Post subject: Newbie question Reply with quote
Hi,

I'm extremely new to this technique and no one else in the lab does it so I don't know where to go to for help, so I hope you all will be kind enough to answer some of my questions.

I've been trying to look around for just how long the probes need to be in order to do RNA ISH and came back with varying answers. One suggested a length greater than 1 KB others say it's fine if it's around 500 bp. Which is it?
PostSun Apr 19, 2009 11:14 pm
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Laoz

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Joined: Apr 13, 2007

Posts: 109

Post subject: Reply with quote
Both 1 Kb and 500 bp are possible good probe. there is no absolute answer.
Laoz
PostSat Apr 25, 2009 2:34 am
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