There are currently, 128 guest(s) and 0 member(s) that are online.
You are Anonymous user. You can register for free by clicking here
Login
Don't have an account yet? You can create one. As a registered user you have some advantages like theme manager, comments configuration and post comments with your name.
I have used AEC as substrate in immunoperoxidase test as its colour is better differentiated. Whether the AEC is having any special affinity to some tissues, such as epithelium, or RBC. If anybody can add something on this matter?
Mon Aug 29, 2005 8:24 am
hrn
Rank: Site Admin
Joined: Sep 27, 2004
Posts: 246
Post subject:
Personally, I prefer DAB. AEC has to be mounted in aqueous mouning medium (less optical quality) and it tends to fade over time. Should be stored in dark.
"Affinity" to tissues, I think is a mattar of endogenous peroxidase, and can be avoided by properly quencheing.
If you prefere red color, NovaRED, wich can be permanently mounted in non-aqueous mounting medium, might be an alternative?
Hogne
Mon Aug 29, 2005 10:39 am
Carl
Rank: Member
Joined: Oct 11, 2004
Posts: 1871
Post subject: I agree with Hogne....
The epithelial " non-specific" staining that you mention you get with epithelium ( as opposed to the rbc-staining).....could it be due to your primary Ab? Or does it occur with all Abs? What type of section prep? Commercial AEC, or homemade?
You cannot post new topics in this forum You cannot reply to topics in this forum You cannot edit your posts in this forum You cannot delete your posts in this forum You cannot vote in polls in this forum
