I have used AEC as substrate in immunoperoxidase test as its colour is better differentiated. Whether the AEC is having any special affinity to some tissues, such as epithelium, or RBC. If anybody can add something on this matter?
Mon Aug 29, 2005 8:24 am
Rank: Site Admin
Joined: Sep 27, 2004
Personally, I prefer DAB. AEC has to be mounted in aqueous mouning medium (less optical quality) and it tends to fade over time. Should be stored in dark.
"Affinity" to tissues, I think is a mattar of endogenous peroxidase, and can be avoided by properly quencheing.
If you prefere red color, NovaRED, wich can be permanently mounted in non-aqueous mounting medium, might be an alternative?
Mon Aug 29, 2005 10:39 am
Joined: Oct 11, 2004
Post subject: I agree with Hogne....
The epithelial " non-specific" staining that you mention you get with epithelium ( as opposed to the rbc-staining).....could it be due to your primary Ab? Or does it occur with all Abs? What type of section prep? Commercial AEC, or homemade?
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