There are currently, 44 guest(s) and 0 member(s) that are online.
You are Anonymous user. You can register for free by clicking here
Login
Don't have an account yet? You can create one. As a registered user you have some advantages like theme manager, comments configuration and post comments with your name.
To provide greater awareness of how easy it is for scientists to label primary antibodies within their own laboratories; Innova Biosciences (Cambridge UK) has launched a new marketing campaign - Go Direct! Rather than struggling with secondary reagents, scientists with the help of Lightning-Link™ are now able to label an antibody directly with minimal hands on time, less than 30 seconds.
Go Direct!
Direct labeling of antibodies greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. The Lightning-Link™ range, the World’s easiest to use DIY antibody labeling kits, consists of over 40 different labels including enzymes, fluorescent proteins/dyes, tandems, biotin and streptavidin -
The Go Direct! campaign will run throughout 2010 with the objective being to increase awareness of how easy it is to directly label antibodies and in doing so, accelerate pioneering science whilst simultaneously saving both money and time!
Seeing is believing! For a timed demonstration video of the Lightning-Link™ antibody labeling process, please visit - Video
"
Posted by admin on Monday, January 18 @ 18:43:48 CET (550 reads) (Read More... | 2168 bytes more | Score: 0)
Technical pitfalls potentially affecting diagnoses in immunohistochemistry
Abstract
Result of the immunohistochemical reactions routinely used in diagnostic surgical pathology should be properly interpreted, since false results, related to technical and interpretative pitfalls may lead to incorrect diagnosis. The main sources of such pitfalls are reviewed, analytically described and related to different steps (fixation, tissue processing and embedding, decalcification, antigen retrieval) which may affect the accuracy of immunohistochemistry. In addition, the presence of endogenous enzyme activity, improper binding of avidin to endogenous biotin, incorrect use of antibodies, chromogen and detection systems, as well as incorrect interpretation may produce unreliable data. The high frequency and extension of such pitfalls make mandatory the use of internal and external controls and adoption of cross-validation programmes. The present study, supported by an extensive review of the related literature, is intended as a guideline leading to proper interpretation of immunohistochemical data, an essential component of the diagnostic process. Experience on the antigen retrieval procedures for different antigens is also presented.
