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Protocols
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Epitope Retrieval
Epitope Retrieval
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Protocol Name Reads - Category Submitted by
Caspase 3 Cleaved in rat FFPWs Cell Signalling 9661 1712   Immunoenzyme carl
 "General" IHC protocol FFPET human tissue, Ole 1590   Immunoenzyme ole
 α-methylacyl-CoA racemase (AMACR) Ventana BenchMark 1242   Immunoenzyme Anonymous
4D4 ( A2B5-like) on cell monolayers 1547   Immunofluorescence carl
A2B5 on Cell monolayers 1791   Immunofluorescence carl
 ABC method for any Antibody : cells and tissue sections. 992   Immunoenzyme carl
 ABC Protocol for Monoclonal Antibodies 8031   Immunoenzyme Anonymous
 ABC Protocol for Polyclonal Antibodies 5814   Immunoenzyme Anonymous
 ABC Protocol for Vibratome Sections 2739   Immunoenzyme admin
 ABC Protocol Monoclonal Antibodies (Frozen Sections) 5145   Immunoenzyme Anonymous
 ABC Protocol Polyclonal Antibodies (Frosen Sections) 4188   Immunoenzyme Anonymous
ACSVL4 (C-15) 1951   Immunoenzyme Anonymous
 Actin (Muscle Actin) Protocol for ES/NexES 4362   Immunoenzyme Admin
 Actin (Smooth Muscle) 1994   Immunoenzyme Anonymous
 Actin (Smooth Muscle) Protocol for Autostainer (Cytospins) 3856   Immunoenzyme Anonymous
Actin alpha Sarcomeric in rat FFPW section 2229   Immunoenzyme carl
Actin alpha: Pan-muscle specific. FFPW sections 4200   Immunoenzyme carl
 Actin:alpha, Smooth muscle -specific FFPW sections (ASMA) 1824   Immunoenzyme carl
Adaptin in FFPW sections of Mouse/rat 2341   Immunoenzyme carl
 Adrenocorticotropin (ACTH) Protocol for ES/NexES 4092   Immunoenzyme Admin
 ALDH1A1: ab23375 241   Epitope Retrieval carl
 ALDH1L1 - ab87117 1210   Epitope Retrieval carl
ALK Protein Protocol for ES/NexES 4057   Immunoenzyme Admin
 ALK-1 Ventana BenchMark XT 2191   Immunoenzyme Anonymous
alpha B crystallin in FFPW sections 1695   Immunoenzyme Carl
alpha B crystallin phospho S45 in FFPW sections 1595   Immunoenzyme Carl
 alpha B crystallin phosphoS59 FFPW sections 1465   Immunoenzyme Carl
 alpha fetoprotein in FFPWS: A8452 283   Epitope Retrieval carl
alpha Synuclein (610787) in Human,Rat,mouse Pwax sections 2081   Immunoenzyme carl
 alpha Synuclein (AB5038) in Human,Rat,mouse Pwax sections 1883   Immunoenzyme carl
 Alpha-1-Antitrypsin Protocol for ES/NexES 4264   Immunoenzyme Admin
Alpha-Sarcoglycan (Adhalin) Protocol for NexES/ES 4618   Immunoenzyme Admin
 Alpha-Smooth Muscle Actin (ASMA) Ventana BenchMark XT 1978   Immunoenzyme Anonymous
Alpha-Synuclein Protocol for ES/NexES 4581   Immunoenzyme Admin
Amyloid A Component Protocol for ES/NexES 3968   Immunoenzyme Admin
Amyloid Beta and ?APP? :SIG-39320 2285   Immunoenzyme carl
Amyloid Beta in FFPW sections: 44-344 1819   Immunoenzyme carl
Amyloid Beta in FFPW sections: 44-348 1712   Immunoenzyme carl
Amyloid Beta in FFPW sections: ab11132 1901   Immunoenzyme carl
 Amyloid beta in FFPW sections: ab12267 1081   Immunoenzyme carl
 Amyloid Beta in FFPW sections: ab14220 1193   Immunoenzyme carl
Amyloid Beta in FFPW sections: ab2539 1829   Immunoenzyme carl
Amyloid Beta: SIG-39142 1883   Immunoenzyme carl
Anti Mouse CD34 (FFPT) 2177   Immunoenzyme Andrew321
Anti mouse CD4 on Mouse Tumour (FFPT) 3945   Immunoenzyme Andrew321
Anti Mouse PECAM-1 (CD31) Mouse Kidney FFPT 4720   Immunoenzyme Andrew321
Anti Mouse VCAM-1 (FFPT) 2066   Immunoenzyme Andrew321
 Anti VWF: ab11713 154   Epitope Retrieval carl
Antigen Retrieval Tris pH 10 using M/waveable pressure cooker 9646   Epitope Retrieval Carl
 Antigen Retrieval: methods overview 1018   Epitope Retrieval carl
 APAAP General Protocol (Mouse Primary) 4991   Epitope Retrieval Admin
 APAAP General Protocol (Rabbit Primary) 4979   Immunoenzyme Admin
APC protein in rat/mouse FFPWs 2705   Immunoenzyme carl
 APLP2 in FFPW sections 1473   Immunoenzyme carl
 Apo E - ab1906 193   Epitope Retrieval carl
 Apolipoprotein D Protocol for ES/NexES 3995   Immunoenzyme Admin
 APP Amyloid precursor protein in FFPW sections of mouse/rat: CT695 1959   Immunoenzyme carl
 APP Amyloid Precursor Protein in Mouse brain: 1565-1 1271   Immunoenzyme carl
Aquaporin in mouse FFPW section 2575   Immunoenzyme carl
 asc: ALX-210-905 5   Epitope Retrieval carl
Aspergillus Protocol for ES/NexES 4248   Immunoenzyme Admin
 ATP synthase in FFPWS: MS507 506   Epitope Retrieval carl
Atrogin-1 in mouse,rat, human FFPW sections 2274   Immunoenzyme carl
B-catenin staining FFPET 5187   Immunoenzyme ole
 Background-free Pax7 Mouse on Mouse 3583   Immunoenzyme TML
 Bcl-2 Oncoprotein Protocol for ES/NexES 4591   Immunoenzyme Admin
Bcl-6 4891   Immunoenzyme Admin
Bcl-6 Protocol for ES/NexES 4603   Immunoenzyme Admin
 bcl-6 Ventana BenchMark XT 2271   Immunoenzyme Anonymous
BCL6 on FFPET 4233   Immunoenzyme ole
 beta III Tubulin for FFPWS: ab18207 696   Epitope Retrieval carl
 beta III Tubulin in cells, frozen and FFPW sections: ab7751 1609   Immunoenzyme carl
beta III Tubulin in FFPW sections: TP1691 2371   Immunoenzyme carl
 Beta III Tubulin: ab78078 1937   Immunoenzyme carl
 Beta tubulin III ( TUJ1) in FFPW section 2796   Immunoenzyme Carl
Beta Tubulin Monoclonal in FFPW tissues of rat, mouse, chick, human 4907   Immunoenzyme Carl
Beta tubulin Polyclonal in FFPWs of human, rat, mouse. 4859   Immunoenzyme Carl
 Beta-Dystroglycan Protocol for NexES/ES 4333   Immunoenzyme Admin
Beta-Sarcoglycan Protocol for NexES/ES 4250   Immunoenzyme Admin
 Biotin:blocking endogenous biotin using in-house reagents 3517   Miscellenous carl
 Blocking Endogenous Peroxidase Activity 728   Miscellenous Anonymous
 Blocking solution for non-specific binding in pwax sections 4028   Immunoenzyme carl
Bob-1 Protocol for ES/NexES 4459   Immunoenzyme Admin
 Brain Lipid Binding Protein in CNS FFPW sections 2566   Immunoenzyme carl
 BrdU 7860   Immunoenzyme Anonymous
 BRDU in FFPE Mouse Tissue 6724   Immunoenzyme Anonymous
 BrdU in FFPWS after Citric acid HIER 2803   Epitope Retrieval carl
BrdU in Pwax sections using HCL/enzyme retrieval: any species 5304   Immunoenzyme carl
Bungarotoxin labelling of mouse neuromuscular junction 4622   Immunofluorescence carl
 C4d Complement 5437   Immunoenzyme Admin
 Cadherin Pan in mouse/rat: CP1901 294   Epitope Retrieval carl
 Cadherin PAN on mouse, rat, human, chick FFPW sections 2253   Immunoenzyme Carl
Calbindin D detection FFPW sections human, rat. mouse, chick: C9848 4289   Immunoenzyme carl
 Calbindin D in FFPW sections: CB38 1271   Immunoenzyme carl
 Calcitonin Protocol for ES/NexES 4346   Immunoenzyme Admin
 Calnexin in FFPW sections 1036   Immunoenzyme carl
Calretinin on FFPET- Cellblock 2686   Immunoenzyme ole
 Calretinin Protocol for Autostainer 4657   Immunoenzyme Admin
 Calretinin Protocol for Autostainer (Cytospins) 4182   Immunoenzyme Anonymous
 Calretinin Ventana BenchMark XT 2060   Immunoenzyme Anonymous

Immunohistochemistry - Antibody - Immunohistochemistry




500 Protocols (5 Pages, 100 Per page)
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Immunohistochemistry - Antibody - Immunohistochemistry

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Forums
Last 20 Forum Messages

3-in-1 solution for retrieval: is it possible home-made?
Last post by ole in Automated Immunohistochemistry on May 10, 2013 at 12:54:36

Antigen degradation in IHC
Last post by invasifspecies in General Immunohistochemistry on Apr 16, 2013 at 20:21:38

Cleaved Caspase-3 drama!
Last post by Carl in General Immunohistochemistry on Apr 07, 2013 at 17:20:25

Tissue Preservation
Last post by Carl in General Immunohistochemistry on Mar 10, 2013 at 14:24:33

CH50 test
Last post by honololo in General Immunohistochemistry on Mar 08, 2013 at 19:22:06

ATPase Staining Problems
Last post by Travical in General Immunohistochemistry on Mar 06, 2013 at 20:15:38

Vectastain ABC kit
Last post by Carl in General Immunohistochemistry on Dec 17, 2012 at 17:17:19

Primary antibody background in IF
Last post by Carl in General Immunohistochemistry on Dec 17, 2012 at 17:14:44

Need help with a miRNA-ISH amplified with TSA-Cy5!
Last post by Natural_Science in General In Situ Hybridization on Nov 29, 2012 at 13:38:05

improved IHC and ISH of older slides and labile proteins
Last post by Carl in General Immunohistochemistry on Nov 16, 2012 at 20:13:34

Fixation protocol for frozen sections using PLP
Last post by Carl in General Immunohistochemistry on Nov 16, 2012 at 05:53:50

IBA-1 background problem
Last post by Carl in General Immunohistochemistry on Oct 26, 2012 at 18:57:48

bubbles and folds between slde and frozen sections
Last post by gise in General Immunohistochemistry on Oct 23, 2012 at 09:05:03

Sections curl during cryosectioning?
Last post by AR in General Immunohistochemistry on Sep 20, 2012 at 20:54:30

Statistical analysis of IHC results (urgent help)
Last post by rai in General Immunohistochemistry on Sep 19, 2012 at 12:53:16

Immunodetection kits
Last post by ole in General Immunohistochemistry on Aug 22, 2012 at 12:27:11

DAB- my homemade recipe
Last post by Carl in General Immunohistochemistry on Aug 17, 2012 at 21:25:53

identifying oblique cut in cryosectioning?
Last post by AR in General Immunohistochemistry on Aug 16, 2012 at 22:18:48

Bubbles forming during hybridization in fresh frozen tissue
Last post by nickd in General In Situ Hybridization on Jul 17, 2012 at 00:16:58

Surfactants in HIER solutions
Last post by ole in General Immunohistochemistry on Jun 21, 2012 at 14:18:23


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Draxin in mouse cerebellum: DP3671

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Old Articles
Wednesday, January 13
· Immunohistochemistry ABC
· Immunohistochemistry ImmPress
· Immunohistochemistry
Tuesday, December 29
· Digital Pathology
Wednesday, December 23
· PLA™ Proximity Ligation Assay technology
· Antibody Pair for PLA
Monday, December 21
· PLA™ Proximity Ligation Assay technology
Thursday, October 22
· Diagnostic Immunohistochemistry
Friday, September 25
· Human Antibody Initiative
Friday, August 28
· Multiple Antigen Labeling Guide

Older Articles
 
Top10 Links
· 1: Anderson Lab In Situ Hybridization Protocols
· 2: Detection of mRNA by in situ hybridization
· 3: DAKO Handbook: Immunohistochemical Staining Methods
· 4: 35S-labeled probe
· 5: DIG-labeled probe
· 6: Detection and amplification of FISH signal
· 7: Chromosome In Situ Hybridization using biotin labeled probes
· 8: In Situ Hybridization
· 9: Chick, mouse, and Xenopus two colour whole mount ISH
· 10: CureVac
 
Molecular Cancer
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·Geisinger Partners With Indivumed for Personalized Medicine Initiative - MarketWatch (press release)

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Immunohistochemistry In Situ Hybridization Immunostaining
Immunohistochemistry Images Tissue staining Pathology
Tumor Markers IHC Reagents Tissue Micro Array
Immunofluorescence Staining Positive Control Slide Polyclonal Antibody
Monoclonal Antibody IHC Staining Protocol In Situ Hybridization Images
Immunohistochemistry Protocol Immunofluorescence Immunohistochemical Staining
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