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Protocols
Immunohistochemistry Staining Protocols
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Category
Epitope Retrieval
Epitope Retrieval
(366)
Immunoenzyme
Immunoenzyme
(623)
Immunofluorescence
Immunofluorescence
(83)
Miscellenous
Miscellenous
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Protocol Name Reads - Category Submitted by
 Nanog in Mouse - AF2729 353   Epitope Retrieval carl
 Nanog: ab14959 186   Epitope Retrieval carl
 Nanog: ab70482 202   Epitope Retrieval carl
 Nanog: ab80892 259   Epitope Retrieval carl
NCAD in Chick e7 pwax section 1037   Immunoenzyme carl
 NCAD in mouse/rat: CM1701 255   Epitope Retrieval carl
 NCAD in rat/mouse: CP1751 248   Epitope Retrieval carl
 NCAD: 610920 234   Epitope Retrieval carl
 NCAM -PSA in rat/mouse FFPW sections MAB5324 930   Immunoenzyme carl
NCAM 5B8 localisation in FFPW section of rat brain 1263   Immunoenzyme carl
NCAM AG1: FFPW sections of rat/mouse 998   Immunoenzyme carl
 NCAM-PSA in rat/mouse FFPW sections - C9672 1113   Immunoenzyme carl
 Nestin in human, rat and mouse paraffin wax section ab5968 1116   Immunoenzyme carl
 Nestin in human/rat/mouse FFPW sections AB5922 991   Immunoenzyme carl
 Nestin in mouse Cor1 cells: Rat 401 DSHB 1672   Immunofluorescence carl
Nestin localisation in human, rat FFPWs DSHB rat401 3070   Immunoenzyme carl
 Nestin localisation in rat FFPW sections sc-20978 1023   Immunoenzyme carl
 Nestin: ab6142 258   Epitope Retrieval carl
 Netrin G1 ligand in FFPW sections: ab31983 1200   Immunoenzyme carl
 NeuN (Neuronal nuclei) in mouse/rat FFPWs: ab77315 996   Immunoenzyme carl
NeuN (Neuronal nuclei) in mouse/rat FFPWs: MAB377 1379   Immunoenzyme carl
 NeuN/FOX3: ab104225 81   Epitope Retrieval carl
 Neuregulin 1, type 1. FFPW sections:human,rat,mouse,zebrafish 1021   Immunoenzyme carl
Neurocan localisation in FFPW section of rat brain 1370   Immunoenzyme carl
 NeuroD2 - ab104430 150   Epitope Retrieval carl
Neurofilament 160 ( RMO ) in FFPWs 2808   Immunoenzyme carl
Neurofilament 160 (NN18) in FFPWs 3540   Immunoenzyme carl
 Neurofilament 160: ab64300 278   Epitope Retrieval carl
Neurofilament associated protein 2849   Immunoenzyme Carl
 Neurofilament L in FFPW sections: NB300-131 629   Immunoenzyme carl
 Neurofilament Protein Protocol for ES/NexES 2758   Immunoenzyme Admin
 Neuron Specific Enolase (NSE) 2937   Immunoenzyme Admin
Neuronal Nuclei (NeuN) Protocol for ES/NexES 4012   Immunoenzyme Admin
 Neuropeptide Y in FFPWS: ab30914 1010   Immunoenzyme carl
Neurotactin in fly embryo FFPWs 2728   Immunoenzyme Carl
 Neutrophil elastase: ab68672 132   Epitope Retrieval carl
NG2 in mouse/rat FFPW sections 1317   Immunoenzyme carl
 NG2: ab129051 27   Epitope Retrieval carl
NGFRp75 in FFPWs:mouse,rat,human ab77286 927   Immunoenzyme carl
NGFRp75 in human, mouse, rat FFPWs ab70481 877   Immunoenzyme carl
 NGFRp75 in rat/mouse FFPWsections: AB1554 908   Immunoenzyme carl
 Nicastrin : ab45425 24   Epitope Retrieval carl
 Nicastrin: ab24741 23   Epitope Retrieval carl
 Nkx-2.5 Cardiac specific homeobox protein Csx in rat E16 FFPW section 1291   Immunoenzyme carl
 nNOS in FFPW sections of Rat/mouse/human/cow - NP2141 983   Immunoenzyme carl
 Nuclear Antigen ( Human-Nucleolar/teleophase) FFPW section 1260   Immunoenzyme carl
Nucleostemin in mouse/Rat brain: ab70346 1035   Immunoenzyme carl
 Nucleostemin in Pwax sections (ab32682) 1306   Immunoenzyme carl
 Nucleostemin in pwax sections (AF1638) 1043   Immunoenzyme carl
 Nucleostemin in rat/mouse Pwax sections: ab14293 1041   Immunoenzyme carl


Immunohistochemistry - Antibody - Immunohistochemistry




Immunohistochemistry - Antibody - Immunohistochemistry

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Forums
Last 20 Forum Messages

3-in-1 solution for retrieval: is it possible home-made?
Last post by ole in Automated Immunohistochemistry on May 10, 2013 at 12:54:36

Antigen degradation in IHC
Last post by invasifspecies in General Immunohistochemistry on Apr 16, 2013 at 20:21:38

Cleaved Caspase-3 drama!
Last post by Carl in General Immunohistochemistry on Apr 07, 2013 at 17:20:25

Tissue Preservation
Last post by Carl in General Immunohistochemistry on Mar 10, 2013 at 14:24:33

CH50 test
Last post by honololo in General Immunohistochemistry on Mar 08, 2013 at 19:22:06

ATPase Staining Problems
Last post by Travical in General Immunohistochemistry on Mar 06, 2013 at 20:15:38

Vectastain ABC kit
Last post by Carl in General Immunohistochemistry on Dec 17, 2012 at 17:17:19

Primary antibody background in IF
Last post by Carl in General Immunohistochemistry on Dec 17, 2012 at 17:14:44

Need help with a miRNA-ISH amplified with TSA-Cy5!
Last post by Natural_Science in General In Situ Hybridization on Nov 29, 2012 at 13:38:05

improved IHC and ISH of older slides and labile proteins
Last post by Carl in General Immunohistochemistry on Nov 16, 2012 at 20:13:34

Fixation protocol for frozen sections using PLP
Last post by Carl in General Immunohistochemistry on Nov 16, 2012 at 05:53:50

IBA-1 background problem
Last post by Carl in General Immunohistochemistry on Oct 26, 2012 at 18:57:48

bubbles and folds between slde and frozen sections
Last post by gise in General Immunohistochemistry on Oct 23, 2012 at 09:05:03

Sections curl during cryosectioning?
Last post by AR in General Immunohistochemistry on Sep 20, 2012 at 20:54:30

Statistical analysis of IHC results (urgent help)
Last post by rai in General Immunohistochemistry on Sep 19, 2012 at 12:53:16

Immunodetection kits
Last post by ole in General Immunohistochemistry on Aug 22, 2012 at 12:27:11

DAB- my homemade recipe
Last post by Carl in General Immunohistochemistry on Aug 17, 2012 at 21:25:53

identifying oblique cut in cryosectioning?
Last post by AR in General Immunohistochemistry on Aug 16, 2012 at 22:18:48

Bubbles forming during hybridization in fresh frozen tissue
Last post by nickd in General In Situ Hybridization on Jul 17, 2012 at 00:16:58

Surfactants in HIER solutions
Last post by ole in General Immunohistochemistry on Jun 21, 2012 at 14:18:23


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Wednesday, January 13
· Immunohistochemistry ABC
· Immunohistochemistry ImmPress
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Tuesday, December 29
· Digital Pathology
Wednesday, December 23
· PLA™ Proximity Ligation Assay technology
· Antibody Pair for PLA
Monday, December 21
· PLA™ Proximity Ligation Assay technology
Thursday, October 22
· Diagnostic Immunohistochemistry
Friday, September 25
· Human Antibody Initiative
Friday, August 28
· Multiple Antigen Labeling Guide

Older Articles
 
Top10 Links
· 1: Anderson Lab In Situ Hybridization Protocols
· 2: Detection of mRNA by in situ hybridization
· 3: DAKO Handbook: Immunohistochemical Staining Methods
· 4: 35S-labeled probe
· 5: DIG-labeled probe
· 6: Detection and amplification of FISH signal
· 7: Chromosome In Situ Hybridization using biotin labeled probes
· 8: In Situ Hybridization
· 9: Chick, mouse, and Xenopus two colour whole mount ISH
· 10: CureVac
 
Molecular Cancer
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