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Protocols
Immunohistochemistry Staining Protocols
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Epitope Retrieval
Epitope Retrieval
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Protocol Name Reads - Category Submitted by
Caspase 3 Cleaved in rat FFPWs Cell Signalling 9661 1717   Immunoenzyme carl
4D4 ( A2B5-like) on cell monolayers 1551   Immunofluorescence carl
A2B5 on Cell monolayers 1795   Immunofluorescence carl
 ABC method for any Antibody : cells and tissue sections. 1014   Immunoenzyme carl
Actin alpha Sarcomeric in rat FFPW section 2237   Immunoenzyme carl
Actin alpha: Pan-muscle specific. FFPW sections 4209   Immunoenzyme carl
 Actin:alpha, Smooth muscle -specific FFPW sections (ASMA) 1830   Immunoenzyme carl
Adaptin in FFPW sections of Mouse/rat 2343   Immunoenzyme carl
 ALDH1A1: ab23375 247   Epitope Retrieval carl
 ALDH1L1 - ab87117 1217   Epitope Retrieval carl
alpha B crystallin in FFPW sections 1698   Immunoenzyme Carl
alpha B crystallin phospho S45 in FFPW sections 1600   Immunoenzyme Carl
 alpha B crystallin phosphoS59 FFPW sections 1467   Immunoenzyme Carl
 alpha fetoprotein in FFPWS: A8452 289   Epitope Retrieval carl
alpha Synuclein (610787) in Human,Rat,mouse Pwax sections 2085   Immunoenzyme carl
 alpha Synuclein (AB5038) in Human,Rat,mouse Pwax sections 1887   Immunoenzyme carl
Amyloid Beta and ?APP? :SIG-39320 2290   Immunoenzyme carl
Amyloid Beta in FFPW sections: 44-344 1824   Immunoenzyme carl
Amyloid Beta in FFPW sections: 44-348 1717   Immunoenzyme carl
Amyloid Beta in FFPW sections: ab11132 1904   Immunoenzyme carl
 Amyloid beta in FFPW sections: ab12267 1088   Immunoenzyme carl
 Amyloid Beta in FFPW sections: ab14220 1201   Immunoenzyme carl
Amyloid Beta in FFPW sections: ab2539 1830   Immunoenzyme carl
Amyloid Beta: SIG-39142 1887   Immunoenzyme carl
 Anti VWF: ab11713 157   Epitope Retrieval carl
Antigen Retrieval Tris pH 10 using M/waveable pressure cooker 9675   Epitope Retrieval Carl
 Antigen Retrieval: methods overview 1026   Epitope Retrieval carl
APC protein in rat/mouse FFPWs 2711   Immunoenzyme carl
 APLP2 in FFPW sections 1477   Immunoenzyme carl
 Apo E - ab1906 196   Epitope Retrieval carl
 APP Amyloid precursor protein in FFPW sections of mouse/rat: CT695 1965   Immunoenzyme carl
 APP Amyloid Precursor Protein in Mouse brain: 1565-1 1274   Immunoenzyme carl
Aquaporin in mouse FFPW section 2579   Immunoenzyme carl
 asc: ALX-210-905 7   Epitope Retrieval carl
 ATP synthase in FFPWS: MS507 514   Epitope Retrieval carl
Atrogin-1 in mouse,rat, human FFPW sections 2284   Immunoenzyme carl
 beta III Tubulin for FFPWS: ab18207 702   Epitope Retrieval carl
 beta III Tubulin in cells, frozen and FFPW sections: ab7751 1617   Immunoenzyme carl
beta III Tubulin in FFPW sections: TP1691 2381   Immunoenzyme carl
 Beta III Tubulin: ab78078 1942   Immunoenzyme carl
 Beta tubulin III ( TUJ1) in FFPW section 2802   Immunoenzyme Carl
Beta Tubulin Monoclonal in FFPW tissues of rat, mouse, chick, human 4916   Immunoenzyme Carl
Beta tubulin Polyclonal in FFPWs of human, rat, mouse. 4872   Immunoenzyme Carl
 Biotin:blocking endogenous biotin using in-house reagents 3530   Miscellenous carl
 Blocking solution for non-specific binding in pwax sections 4034   Immunoenzyme carl
 Brain Lipid Binding Protein in CNS FFPW sections 2571   Immunoenzyme carl
 BrdU in FFPWS after Citric acid HIER 2812   Epitope Retrieval carl
BrdU in Pwax sections using HCL/enzyme retrieval: any species 5309   Immunoenzyme carl
Bungarotoxin labelling of mouse neuromuscular junction 4631   Immunofluorescence carl
 Cadherin Pan in mouse/rat: CP1901 298   Epitope Retrieval carl
 Cadherin PAN on mouse, rat, human, chick FFPW sections 2257   Immunoenzyme Carl
Calbindin D detection FFPW sections human, rat. mouse, chick: C9848 4295   Immunoenzyme carl
 Calbindin D in FFPW sections: CB38 1277   Immunoenzyme carl
 Calnexin in FFPW sections 1039   Immunoenzyme carl
 Caspase 3 Cleaved in FFPW sections Abcam ab2302 2153   Immunoenzyme carl
 Caspase 3 Cleaved in FFPW sections Biocare 2322   Immunoenzyme carl
 Caspase 3 cleaved in FFPW sections: Cell Signalling 9661 2289   Immunoenzyme carl
caspase 6 Active/cleaved in FFPW sections: human/rat/mouse/Marmoset. ab52295 1995   Immunoenzyme carl
 CCR2: ab32144 105   Epitope Retrieval carl
 CD10 for FFPWS: AF2736 726   Immunoenzyme carl
 Cd117 C-kit FFPW sections 2090   Immunoenzyme carl
CD133 localisation in FFPWs of rat adult brain 3565   Immunoenzyme Carl
 CD20 in Human/Marmoset FFPW sections: ab78237 1144   Immunoenzyme carl
 CD20 in Human/Marmoset FFPW sections: cloneEP459Y 1038   Immunoenzyme carl
 CD20 in rat and mouse FFPWS: sc-7735 865   Immunoenzyme carl
 CD20cy localisation in human/\Monkey tonsil FFPWS 1912   Immunoenzyme carl
CD3 - A0452 3360   Immunoenzyme carl
CD3 Rabbit monoclonal in mouse FFPW section 3800   Immunoenzyme Carl
 CD45 - 14-0459 161   Epitope Retrieval carl
 CD45 in FFPWS rat, mouse, human, Marmoset: ab10558 653   Immunoenzyme carl
 CD45: M0701 15   Epitope Retrieval carl
CD56 ERIC NCAM on FFPWs 3218   Immunoenzyme Carl
CD56 H28 NCAM on FFPW sections of mouse 3486   Immunoenzyme Carl
CD57 localisation in FFPWs of mouse, rat and chick sections 3472   Immunoenzyme Carl
 CD64: 305015 52   Immunoenzyme carl
 CD68 in Human tissue: ab844 170   Epitope Retrieval carl
 CD68 in rat FFPWS: ab31630 768   Immunoenzyme carl
 CD79a in Human, Rat, Marmoset FFPWS: ab3121 421   Immunoenzyme carl
 CGRP :AB15360 210   Epitope Retrieval carl
 CGRP: ab36001 168   Epitope Retrieval carl
 CGRP: ab47027 170   Epitope Retrieval carl
 CGRP: BML-CA1137 27   Epitope Retrieval carl
ChAT in FFPW sections of human/mouse/rat:AB144P 1347   Immunoenzyme carl
 Chromogranin A in FFPWs: ab15160 1277   Immunoenzyme carl
 Chromogranin A in FFPWs: ab45138 1313   Immunoenzyme carl
 Chromogranin A in FFPWs: ab45179 1257   Immunoenzyme carl
 Chromogranin A in FFPWs: ab85554 1244   Immunoenzyme carl
 Chromogranin A in FFPWs: M0869 1303   Immunoenzyme carl
Clara cells 1611   Immunoenzyme carl
 Claudin 11: ab53041 458   Epitope Retrieval carl
Claudin 3: RB 9251 1855   Immunoenzyme carl
 Claudin 4 : 32-9400 82   Epitope Retrieval carl
Claudin 5: RB 9243 1797   Immunoenzyme carl
 Claudin 7: 10284 1572   Immunoenzyme carl
 Collagen Type I in FFPW & Frozen sections: ab34710 606   Epitope Retrieval carl
 Complex IV subunit I in FFPWS: MS404 551   Epitope Retrieval carl
 Complex IV subunit II in FFPWS - MS405 538   Epitope Retrieval carl
 Connexin 43 in FFPW sections: Mouse, Rat, Marmoset, Human: ab11370 1214   Immunoenzyme carl
 CRABP1: ab2816 63   Epitope Retrieval carl
 Crystallin alpha B pS59 on FFPW sections of Human, mouse, rat tissues 1387   Immunoenzyme carl

Immunohistochemistry - Antibody - Immunohistochemistry




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Immunohistochemistry - Antibody - Immunohistochemistry

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Forums
Last 20 Forum Messages

3-in-1 solution for retrieval: is it possible home-made?
Last post by ole in Automated Immunohistochemistry on May 10, 2013 at 12:54:36

Antigen degradation in IHC
Last post by invasifspecies in General Immunohistochemistry on Apr 16, 2013 at 20:21:38

Cleaved Caspase-3 drama!
Last post by Carl in General Immunohistochemistry on Apr 07, 2013 at 17:20:25

Tissue Preservation
Last post by Carl in General Immunohistochemistry on Mar 10, 2013 at 14:24:33

CH50 test
Last post by honololo in General Immunohistochemistry on Mar 08, 2013 at 19:22:06

ATPase Staining Problems
Last post by Travical in General Immunohistochemistry on Mar 06, 2013 at 20:15:38

Vectastain ABC kit
Last post by Carl in General Immunohistochemistry on Dec 17, 2012 at 17:17:19

Primary antibody background in IF
Last post by Carl in General Immunohistochemistry on Dec 17, 2012 at 17:14:44

Need help with a miRNA-ISH amplified with TSA-Cy5!
Last post by Natural_Science in General In Situ Hybridization on Nov 29, 2012 at 13:38:05

improved IHC and ISH of older slides and labile proteins
Last post by Carl in General Immunohistochemistry on Nov 16, 2012 at 20:13:34

Fixation protocol for frozen sections using PLP
Last post by Carl in General Immunohistochemistry on Nov 16, 2012 at 05:53:50

IBA-1 background problem
Last post by Carl in General Immunohistochemistry on Oct 26, 2012 at 18:57:48

bubbles and folds between slde and frozen sections
Last post by gise in General Immunohistochemistry on Oct 23, 2012 at 09:05:03

Sections curl during cryosectioning?
Last post by AR in General Immunohistochemistry on Sep 20, 2012 at 20:54:30

Statistical analysis of IHC results (urgent help)
Last post by rai in General Immunohistochemistry on Sep 19, 2012 at 12:53:16

Immunodetection kits
Last post by ole in General Immunohistochemistry on Aug 22, 2012 at 12:27:11

DAB- my homemade recipe
Last post by Carl in General Immunohistochemistry on Aug 17, 2012 at 21:25:53

identifying oblique cut in cryosectioning?
Last post by AR in General Immunohistochemistry on Aug 16, 2012 at 22:18:48

Bubbles forming during hybridization in fresh frozen tissue
Last post by nickd in General In Situ Hybridization on Jul 17, 2012 at 00:16:58

Surfactants in HIER solutions
Last post by ole in General Immunohistochemistry on Jun 21, 2012 at 14:18:23


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· 1: Anderson Lab In Situ Hybridization Protocols
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· 6: Detection and amplification of FISH signal
· 7: Chromosome In Situ Hybridization using biotin labeled probes
· 8: In Situ Hybridization
· 9: Chick, mouse, and Xenopus two colour whole mount ISH
· 10: CureVac
 
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