Immunohistochemistry - In Situ Hybridization

Prolactin Staining Protocol

Clone: Polyclonal
Dilution: 1:1000
Producer: Dako
Catnr.: A0569

Pretreatment/Epitope Retrieval

No pretreatment required, but higher dilution (1:5000) of primary antibody can be used after HIER in Tris-EDTA pH 9

Detection/Procedure

ImmPRESS Staining Protocol for Rabbit Primary Antibodies

Reagents/Solutions

ImmPRESS Anti-Rabbit Ig (peroxidase) Kit, Vector Laboratories Catalog No. MP-7401
DAB Substarate
Wash Buffer

Additional Information

Prolactin is an adenohypophyseal polypeptide hormone produced in cells of the anterior pituitary gland of most mammals.
Prolactin producing cells constitute about 17% of all normal human anterior pituitary cells.
Elevated counts of these cells have been observed in pregnant women, newborns and in multiparous women.

Positive control tissue: Pituitary gland

Images of Prolactin in immunohistochemistry Image Gallery

Contributed by: Anonymous on Tuesday, February 17. 2009 at 16:07:10

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