Autofluorescence after Glycine
(Glycine)
Tried out two of recommended pretreatments for supressing autofluorescence. Got useful results. Have posted pics. Can't seem do supress rbcs tho. Any suggestions? Three slides microwave- pressure cooked in citric acid soln, pH6. Then into either Glycine, Na Borohydride or dist water, 30mins RT. Then anti NF160, 60mins RT. Then Alexa 594 anti rabbit IgG, 30mins, RT. Mouse gut, E18. Can clearly see nerve trunk and developing enteric plexus in glycine pretreated section