OX42 (CD11b/c equivalent)
Adult rat liver. Anti- OX42.
P.wax section, after Citric acid HIER.Even at a low dilution factor of 1/100, I do not detect any Kupffer cell positivity, yet see non-specific cytoplasmic staining of Hepatocytes.
Section shows a Portal area.
Abcam ab1211, OX42.Std stABCpx detection. Biotin blocking was carried out, prior to primary Ab incubation (I incubated this section at the same time as the MRC OX-6 and OX6 Abs....clearly demonstrating that it is not endogenous biotin that is detected.