PCNA (Tyramide)
Pwax section. Human PM brain. PCNA staining of nuclei using Tyramide amplification. Texas red fluorochrome.
Section incubated with anti PCNA, then goatanti mouse Igs-biotinylated. Then stABCpx, then Tyramide, then stAvidin-Texas red containing Hoechst ( shown separately). Without tyramide amplification I could not get such good signal:noise.